Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13SubstrateUniRule annotation1
Binding sitei46SubstrateUniRule annotation1
Binding sitei66SubstrateUniRule annotation1
Active sitei75Proton donorUniRule annotation1
Binding sitei170SubstrateUniRule annotation1
Sitei172Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei203SubstrateUniRule annotation1
Sitei221Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei230Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciACIT397945:G1G7T-806-MONOMER
UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:Aave_0799
OrganismiAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
Taxonomic identifieri397945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
Proteomesi
  • UP000002596 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000118251 – 292Diaminopimelate epimeraseAdd BLAST292

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi397945.Aave_0799

Structurei

3D structure databases

ProteinModelPortaliA1TKA9
SMRiA1TKA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 77Substrate bindingUniRule annotation2
Regioni221 – 222Substrate bindingUniRule annotation2
Regioni231 – 232Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG091H01QC

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A1TKA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIRFTKMQG AGNDFVVLDE TQGRLGLTPA QYRYLADRHF GVGADQILTV
60 70 80 90 100
RPSPAEGIDF EYVIHNADGG EVEQCGNGAR CFARYVRDKG LTDRDTIRVQ
110 120 130 140 150
TLAGVIAPRL TPDGRVTVDM GRPRFDPAEV PFDASGLAPA ARGAGQTWPL
160 170 180 190 200
ALGAGADAQV VDVAVVSMGN PHAVQLVGDV DAAPVAHTGP LIEGHARFPQ
210 220 230 240 250
RVNAGYLQIE DRARVRLRVY ERGAGETLAC GTGACAAVAA GIRLGLLDAR
260 270 280 290
VDVRTRGGLL TIAWSGDPSE SVFMTGPATT VFEGQIDIPD AP
Length:292
Mass (Da):30,871
Last modified:February 6, 2007 - v1
Checksum:iE064C0B1D72278D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000512 Genomic DNA Translation: ABM31397.1
RefSeqiWP_011793957.1, NC_008752.1

Genome annotation databases

EnsemblBacteriaiABM31397; ABM31397; Aave_0799
KEGGiaav:Aave_0799

Similar proteinsi

Entry informationi

Entry nameiDAPF_ACIAC
AccessioniPrimary (citable) accession number: A1TKA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health