Reviewed, UniProtKB/Swiss-Prot A1TJR8 (GLPK_ACIAC)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Aave_0602

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Glycerol kinase HAMAP MF_00186		PRO_1000098708
Regions
	Nucleotide binding	421 – 425	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	264	1	ATP By similarity
	Binding site	316	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TJR8-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 1AEC9DFBD02CEDA8	FASTA	506	53,912
	10 20 30 40 50 60 MTYLLALDQG TSSSRSIVFD ERGHIVAQAQ QELPQIYPQP GWVEHDPVDI WRTQIATARQ 70 80 90 100 110 120 ALAQANIGPG DVRALGITNQ RETTVLWNRR TGQPVHHAIV WQDRRAEPLC AELREQGHEP 130 140 150 160 170 180 MIQERTGLRI DAYFSATKLR WLLDQVPGAR AAAEAGELAF GTVDSWLIWQ LTGGKVHVTD 190 200 210 220 230 240 VSNASRTMLF NVHSNDWDDD LLALLRIPRK LLPRVQPSAS DFGATDAALL GGAIPIGGVA 250 260 270 280 290 300 GDQQSALFGQ ACFTAGMAKN TYGTGCFMLM HTGSSFQTSR NGLLTTSAAQ IAPRTGAGHG 310 320 330 340 350 360 APEPAFAMEG SVFVGGAVVQ WLRDGLRAIS SSSEVQSLAE SVPDSGGVMM VPAFTGLGAP 370 380 390 400 410 420 YWKPDARGTI TGLTRGTTIA HIARAALESI AYQSAALLQA MSRDAVAAGG APVSELRVDG 430 440 450 460 470 480 GACVNDLLMQ FQADLLGIPV VRPAVIETTA LGAAYLAGLS SGVYAGTEAL SALWRAERRF 490 500 LPTLSAARAQ ECMARWEHAV RQAALD

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM31206.1.
RefSeq	YP_968980.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TJR8.
Genome annotation databases
GeneID	4668657.
GenomeReviews	Gene locus Aave_0602 in contig CP000512_GR.
KEGG	aav:Aave_0602.
NMPDR	fig|397945.5.peg.518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RQTSGIC.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ACIAC

Accession

Primary (citable) accession number: A1TJR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)