Reviewed, UniProtKB/Swiss-Prot A1TIS2 (ALR_ACIAC)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	Aave_0253

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Alanine racemase HAMAP MF_01201		PRO_1000065965
Sites
	Active site	35	1	Proton acceptor; specific for D-alanine By similarity
	Active site	257	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	35	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TIS2-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 657665D567FBFF35	FASTA	369	39,526
	10 20 30 40 50 60 MPRPIQATIH TAALQQNLAR ARAAAPDARV WAVVKANAYG HGIERAFEGL RGADGFALLD 70 80 90 100 110 120 LGEAERVRQL GWRGPILLLE GVFEPRDLEL CSRLSLWHAV HCDEQIDWLS AHKTHAPHRV 130 140 150 160 170 180 FLKMNSGMNR LGFPPARYRA AWARLNALPQ VDEISFMTHF SDADGPRGIA HQIDAFCTAT 190 200 210 220 230 240 QDLPGERTVS NSAATLRHAG GDARVRGDWV RAGIVLYGSA ADHPEHTAAH WGLAPTMTLS 250 260 270 280 290 300 ARIIGTQQLQ AGDTVGYGSS FTADRPMTIG VVACGYADGY PRHAPTGTPV LVNGVRTRTV 310 320 330 340 350 360 GRVSMDMITV DLTPLHEAGV EAGVGSEATL WGRASCGAVL CIDEVAQAAG TVGYELMCAL APRVPVVVD

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM30860.1.
RefSeq	YP_968634.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TIS2.
Genome annotation databases
GeneID	4667043.
GenomeReviews	Gene locus Aave_0253 in contig CP000512_GR.
KEGG	aav:Aave_0253.
NMPDR	fig|397945.5.peg.221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SVELWGD.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_ACIAC

Accession

Primary (citable) accession number: A1TIS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)