A1TG67 (A1TG67_MYCVP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Mvan_5396

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	955 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A1TG67 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D629A23FF312DAC0	FASTA	955	104,501
	10 20 30 40 50 60 MADEERGSGK NGAEPRRGNG SSVRRLVIVE SPTKARKIAG YLGSNYIVES SRGHIRDLPR 70 80 90 100 110 120 NAADVPAKYK SEPWARLGVN VEHNFEPLYI ISPDKKSTVA DLKDKLKNVD ELYLATDGDR 130 140 150 160 170 180 EGEAIAWHLL ETLKPRIPVK RMVFHEITEP AIRAAAEDPR DLDNDLVDAQ ETRRILDRLY 190 200 210 220 230 240 GYEVSPVLWK KVAPKLSAGR VQSVATRIIV QRERERMAFR SAGYWDVTAE LDASVSDAQA 250 260 270 280 290 300 SPPTFVAKLN TVDGRRVATG RDFDSLGAVR KPDEVLVLDE AAANALATGL RGAQLAVSSV 310 320 330 340 350 360 EQKPYTRRPY APFMTSTLQQ EAGRKLRFTS ERTMSIAQRL YENGYITYMR TDSTTLSQSA 370 380 390 400 410 420 IDAARNQARQ LYGEEYVHPT PRQYTRKVKN AQEAHEAIRP AGDVFQTPGQ LHSQLDTDEF 430 440 450 460 470 480 RLYELIWQRT VASQMADARG TTLSLRIAGA APATTLGGGT ASDVQVVFNA SGRTITFPGF 490 500 510 520 530 540 LKAYVESIDD LAGGEADDAE SRLPNLTQGQ RVDAKGLTAD GHTTSPPARY TEASLIKALE 550 560 570 580 590 600 DLGIGRPSTY SSIIKTIQDR GYVHKKGSAL VPSWVAFAVI GLLEQHFGRL VDYDFTAAME 610 620 630 640 650 660 DELDEIAAGH ERRTNWLNNF YFGGEHGADG SIARSGGLKK LVGGNLEEID AREVNSIKLF 670 680 690 700 710 720 DDAEGRAVNV RVGRNGAYLE RMVADPDNPG ELKPQRANLK DELTPDELTL ELAEKLFATP 730 740 750 760 770 780 QEGRSLGVDP ATGHEIVAKD GRYGPYVTEV LPEPPDEGEA GATAKKGKKP TGPKPRTGSL 790 800 810 820 830 840 LRSMDLETVT LEDALRLLSL PRVVGVDPAS GEEITAQNGR YGPYLKRGTD SRSLATEEQM 850 860 870 880 890 900 FDITLEEALK IYAEPKRRGR QGAATPPLRE LGVDPVSEKP MVIKDGRFGP YVTDGETNAS 910 920 930 940 950 LRKGDDVLSI TDARASELLA DRRARGPVKK KAVKKAPAKK TPAKKTAAKK AAKKA

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM16167.1.
RefSeq	YP_956173.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1TG67.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TG67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000080519; EBMYCP00000078472; EBMYCG00000080514.
GeneID	4648076.
GenomeReviews	Gene locus Mvan_5396 in contig CP000511_GR.
KEGG	mva:Mvan_5396.
PATRIC	18189207. VBIMycVan31953_5497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000016895.
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1TG67_MYCVP

Accession

Primary (citable) accession number: A1TG67

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)