A1TFL3 (OTSA_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trehalose-phosphate synthase
Short name=TPS
EC=2.4.1.-

Alternative name(s):
Trehalose-6-phosphate synthase

Gene names

Name:	otsA
Ordered Locus Names:	Mvan_5192

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate By similarity.
Catalytic activity	Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Trehalose-phosphate synthase		PRO_0000348925
Regions
	Region	42 – 43	2	NDP-glucose binding By similarity
	Region	399 – 403	5	NDP-glucose binding By similarity
Sites
	Binding site	22	1	Glucose-6-phosphate By similarity
	Binding site	100	1	Glucose-6-phosphate By similarity
	Binding site	154	1	Glucose-6-phosphate By similarity
	Binding site	296	1	NDP-glucose By similarity
	Binding site	301	1	NDP-glucose By similarity
	Binding site	334	1	Glucose-6-phosphate By similarity
	Site	109	1	Involved in alpha anomer selectivity By similarity
	Site	179	1	Involved in alpha anomer selectivity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TFL3 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 2F76B7B78E911B3F	FASTA	491	55,034
	10 20 30 40 50 60 MAPQSGPEAR SGGADFVVVA NRLPIDMVRR ADGTTEFKRS PGGLVTALEP LLRRRHGAWI 70 80 90 100 110 120 GWPGVPEDAD DPNAATEPIE QDGMTLVPVR LSSEDVAEYY EGFSNATLWP LYHDVIVKPI 130 140 150 160 170 180 YHREWWDRYV DVNRRFAEAT AHTAAEGATV WVQDYQLQLV PKMLRMLRPD LTIGFFLHIP 190 200 210 220 230 240 FPPVELFMQM PWRTEIIEGL LGADLVGFHL PGGAQNFLIL ARRLIGATTS RGNVGVRSRF 250 260 270 280 290 300 GEVQFGFRTV KVGAFPISID SAELDQHARS RATRQRAKEI RAELGNPRKI LLGVDRLDYT 310 320 330 340 350 360 KGIDVRLRAF SELLEEGRID PEDTVLVQLA TPSRERVESY VAMREDIERQ VGHVNGEFGE 370 380 390 400 410 420 VGHPVLHYLH RPIPREDLVA FFVAADVMLV TPLRDGMNLV AKEYVACRHD LGGALVLSEF 430 440 450 460 470 480 TGAAAELRQA YLTNPHHIEG VKDAIEAALT QAPEEGRRRM RAMRRQVLAH DVDRWARSFL 490 DALASKEPVE G

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM15963.1.
RefSeq	YP_955969.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1TFL3.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TFL3.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000077525; EBMYCP00000075478; EBMYCG00000077520.
GeneID	4645709.
GenomeReviews	Gene locus Mvan_5192 in contig CP000511_GR.
KEGG	mva:Mvan_5192.
PATRIC	18188795. VBIMycVan31953_5292.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0380.
GeneTree	EBGT00050000016525.
HOGENOM	HBG559076.
OMA	EYGRKEM.
PhylomeDB	A1TFL3.
ProtClustDB	CLSK872185.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_5192-MONOMER.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. [Graphical view]
KO	K00697.
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTSA_MYCVP

Accession

Primary (citable) accession number: A1TFL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program