A1TEH2 (ISPE_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Mvan_4799

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335731
Regions
	Nucleotide binding	110 – 120	11	ATP Potential
Sites
	Active site	25	1	By similarity
	Active site	152	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TEH2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6164B5EBBFDDDD10	FASTA	322	33,023
	10 20 30 40 50 60 MSARDGNTAS EWVPTGSVTV RVPGKVNLYL DVGDRRDDGY HELTTVFHAV SLLDEVTVRT 70 80 90 100 110 120 ADILSLEMTG EGAESLPTDE RNLAWRAAEL MADHVGRSPD VAISIEKTIP VAGGMAGGSA 130 140 150 160 170 180 DAAAVLVAMN SLWELGVPRR DLHALAAQLG SDVPFALHGG TALGTGRGEE LATVLTRNTF 190 200 210 220 230 240 HWVLAFSPGG LSTAKVFAEI DRLRAEEDRT LPPRLESPEP VLAALASGDP AQLAPLLGND 250 260 270 280 290 300 LQPAALSLDS TLRRTLRAGV DAGALAGVVS GSGPTCAFLC GSAGAAVDVG TELAGAGVCR 310 320 TVRVASGPVQ GARVVPSPSN AG

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM15572.1.
RefSeq	YP_955578.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1TEH2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TEH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000077971; EBMYCP00000075924; EBMYCG00000077966.
GeneID	4646089.
GenomeReviews	Gene locus Mvan_4799 in contig CP000511_GR.
KEGG	mva:Mvan_4799.
PATRIC	18187975. VBIMycVan31953_4889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
GeneTree	EBGT00050000016146.
HOGENOM	HBG734593.
OMA	KINLHLG.
PhylomeDB	A1TEH2.
ProtClustDB	PRK03188.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_4799-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_MYCVP

Accession

Primary (citable) accession number: A1TEH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program