A1TE06 (ISPH_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Mvan_4631

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021141

Sequences

	Sequence		Length	Mass (Da)
	A1TE06 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 9E3CFB2E96959AB9	FASTA	337	36,261
	10 20 30 40 50 60 MPPTVNMGIP GASRTALGSV AGDVTGKRVL LAEPRGYCAG VDRAVETVER ALEKHGAPIY 70 80 90 100 110 120 VRHEIVHNRY VVDTLAKAGA IFVEQTDEVP EGAIVVFSAH GVAPTVHEEA AARHLRTIDA 130 140 150 160 170 180 TCPLVTKVHN EAKRFARDDY DILLVGHEGH EEVVGTAGEA PDHVQVVDNP DAVDKVTVRD 190 200 210 220 230 240 PNKVIWLSQT TLSVDETMET VRRLREKFPT LQDPPSDDIC YATQNRQVAV KAMAPECELV 250 260 270 280 290 300 IVVGSKNSSN SVRLVEVALG AGASAAHLVD YADDIDPAWF DGVTTVGVTS GASVPEILVR 310 320 330 GVLDRLAEHG YDVVQPVTTA NETLVFALPR EIRPARQ

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM15406.1.
RefSeq	YP_955412.1. NC_008726.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TE06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000079079; EBMYCP00000077032; EBMYCG00000079074.
GeneID	4646648.
GenomeReviews	Gene locus Mvan_4631 in contig CP000511_GR.
KEGG	mva:Mvan_4631.
PATRIC	18187619. VBIMycVan31953_4713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
GeneTree	EBGT00050000016858.
HOGENOM	HBG335228.
OMA	AMAPECD.
PhylomeDB	A1TE06.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_4631-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_MYCVP

Accession

Primary (citable) accession number: A1TE06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program