A1TD14 (RNPH_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Mvan_4287

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Ribonuclease PH HAMAP MF_00564		PRO_1000024836

Sequences

	Sequence		Length	Mass (Da)
	A1TD14 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: F891CB4AD1E1A794	FASTA	260	27,466
	10 20 30 40 50 60 MSRREDGRLD DELRPVTITR GFTTHPAGSV LVEFGQTRVM CTASVTEGVP RWRKGSGQGW 70 80 90 100 110 120 LTAEYAMLPA ATHERSGRES VKGRVGGRTQ EISRLVGRSL RACIDLAALG ENTIAIDCDV 130 140 150 160 170 180 LQADGGTRTA AITGAYVALA DAVTYLAAAG KLSDPRPLSC AIAAVSVGVV DGRIRVDLPY 190 200 210 220 230 240 TEDSRAEVDM NVVATDTGTL VEIQGTGEGA TFPRSTLDKM LDAAMAACDQ LFEIQRAALE 250 260 LPYPGVLPEP GEPAKKAFGS

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM15064.1.
RefSeq	YP_955070.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1TD14.
SMR	A1TD14. Positions 3-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TD14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000078063; EBMYCP00000076016; EBMYCG00000078058.
GeneID	4648389.
GenomeReviews	Gene locus Mvan_4287 in contig CP000511_GR.
KEGG	mva:Mvan_4287.
PATRIC	18186901. VBIMycVan31953_4358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
GeneTree	EBGT00050000017525.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	A1TD14.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_4287-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_MYCVP

Accession

Primary (citable) accession number: A1TD14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program