Reviewed, UniProtKB/Swiss-Prot A1TCX1 (MHPB_MYCVP)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	Mvan_4244

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337660
Sites
	Active site	118	1	Proton donor By similarity
	Active site	182	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TCX1-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: E05DD6995015778B	FASTA	316	33,277
	10 20 30 40 50 60 MAKSQMALCC MSHSPLLNLP GPAQELLDDI EGAIAAAREF VAAFDPDLVV TFSPDHYNGF 70 80 90 100 110 120 FYRAMPPFCV GTAAAGVGDY GTYQGPLDVP ADLAIDCARA VLDSDVDVAV SAAMDVDHGT 130 140 150 160 170 180 VQPLQKLFGD ATAKPVIPVF VNSVATPLGP MRRVRALGAA VGTHLAGLGK RVLVIGSGGL 190 200 210 220 230 240 SHDPPVPTLA TAPPAALDRI VRGVPMTTEQ RQARQAAVIE AAREFASGQG ALAPLNPDWD 250 260 270 280 290 300 RAFLDLLDNG RLAEVDSWDN RWIAEQAGNS AHEVRTWVAA FAALAAQGKY ETGNRYYRAA 310 PELIAGFAIR TAVCTS

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000511 Genomic DNA. Translation: ABM15021.1. Different initiation.
RefSeq	YP_955027.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4648952.
GenomeReviews	Gene locus Mvan_4244 in contig CP000511_GR.
KEGG	mva:Mvan_4244.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_MYCVP

Accession

Primary (citable) accession number: A1TCX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 17 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)