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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601ZincUniRule annotation
Binding sitei75 – 751Cysteinyl adenylateUniRule annotation
Binding sitei244 – 2441Cysteinyl adenylateUniRule annotation
Metal bindingi248 – 2481ZincUniRule annotation
Metal bindingi273 – 2731ZincUniRule annotation
Binding sitei300 – 3001Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMVAN350058:GIWR-3503-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Mvan_3478
OrganismiMycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Taxonomic identifieri350058 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000009159 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400470Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi350058.Mvan_3478.

Structurei

3D structure databases

ProteinModelPortaliA1TAS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 634Cysteinyl adenylate bindingUniRule annotation
Regioni98 – 1003Cysteinyl adenylate bindingUniRule annotation
Regioni266 – 2683Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi62 – 7211"HIGH" regionUniRule annotationAdd
BLAST
Motifi204 – 2096"ERGGDP" regionUniRule annotation
Motifi306 – 3105"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiARHYVHT.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

A1TAS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQPSDLSHT SMSRVEAMHS WSAPNVPALP GRGPQLRLYD SADRQVRPVA
60 70 80 90 100
PGATATMYVC GITPYDATHL GHAATYLTFD LVHRLWRDSG HDVHYVQNIT
110 120 130 140 150
DVDDPLFERA QRDGIGWREL GDRETELFRE DMAALRVLPP RDYVAATEAI
160 170 180 190 200
AEVVELVEKM LAAGAAYVVD DPQYPDVYFR ADATVQFGYE SGYDRDTMLA
210 220 230 240 250
LFAERGGDPD RPGKSDPLDA LLWLAERPGE PSWPSPFGPG RPGWHVECAA
260 270 280 290 300
IALSRIGTGL DIQGGGSDLI FPHHEFSAAH AECVTGERRF ARHYVHAGMI
310 320 330 340 350
GWDGHKMSKS RGNLVLVSQL RRDGVDPAAI RLGLFAGHYR DDRFWSPAVL
360 370 380 390 400
DEALARLHRW RSATALAGAP DATDVVARVR RYLADDLDTP KALAALDGWA
410 420
TDALTYGGHD ATAGTTVATA VDALLGIAL
Length:429
Mass (Da):46,870
Last modified:February 6, 2007 - v1
Checksum:iABCACD53F182746B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000511 Genomic DNA. Translation: ABM14273.1.

Genome annotation databases

EnsemblBacteriaiABM14273; ABM14273; Mvan_3478.
KEGGimva:Mvan_3478.
PATRICi18185261. VBIMycVan31953_3547.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000511 Genomic DNA. Translation: ABM14273.1.

3D structure databases

ProteinModelPortaliA1TAS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350058.Mvan_3478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM14273; ABM14273; Mvan_3478.
KEGGimva:Mvan_3478.
PATRICi18185261. VBIMycVan31953_3547.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiARHYVHT.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciMVAN350058:GIWR-3503-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 7251 / PYR-1.

Entry informationi

Entry nameiMSHC_MYCVP
AccessioniPrimary (citable) accession number: A1TAS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: February 6, 2007
Last modified: January 20, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.