A1TAB5 (SYFA_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Mvan_3317

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000006863
Sites
	Metal binding	265	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TAB5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4D290A29FE765B0B	FASTA	347	38,055
	10 20 30 40 50 60 MAEQPVDLSD EALAQAVSAA SRAFDQASTL DELAKAKTEH LGDRSPIALA RQALGSLPKT 70 80 90 100 110 120 DRADAGKRVN VARTQAQAGY DDRLAALRAE RDAEVLVAER IDVTLPSTRQ PVGARHPITI 130 140 150 160 170 180 LAENVADTFV AMGWELAEGP EVETEQFNFD ALNFPPDHPA RSEQDTFHIA PDGSRQVLRT 190 200 210 220 230 240 HTSPVQVRTL LERELPVYVI SIGRTFRTDE LDATHTPVFH QVEGLAVDKG LTMAHLRGTL 250 260 270 280 290 300 DAFARAQFGP QGRTRFRPHF FPFTEPSAEV DIWFPNKKGG PGWVEWGGCG MVNPNVLRAC 310 320 330 340 GIDPEVYSGF AFGMGLERTL QFRNGIPDMR DMVEGDVRFS LPFGVGA

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM14115.1.
RefSeq	YP_954121.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1TAB5.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TAB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000081835; EBMYCP00000079788; EBMYCG00000081830.
GeneID	4644514.
GenomeReviews	Gene locus Mvan_3317 in contig CP000511_GR.
KEGG	mva:Mvan_3317.
PATRIC	18184921. VBIMycVan31953_3378.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000016426.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	A1TAB5.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_3317-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_MYCVP

Accession

Primary (citable) accession number: A1TAB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program