Reviewed, UniProtKB/Swiss-Prot A1T9P9 (MASZ_MYCVP)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Mvan_3097

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Malate synthase G HAMAP MF_00641		PRO_1000056912
Sites
	Active site	345	1	Proton acceptor By similarity
	Active site	639	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1T9P9-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8A57EC7050B20332	FASTA	734	79,327
	10 20 30 40 50 60 MTDRVTVGNL RVAPVLYDFI NNEALPGTDI DPDTFWSGVD KVVADLTPRN QDLLARRDDL 70 80 90 100 110 120 QAQIDRWHRA RVIGGFEPED YKQFLTDIGY LEPEPADFSI TTAGVDDEIT TTAGPQLVVP 130 140 150 160 170 180 ILNARFALNA ANARWGSLYD ALYGTDVISD EGGAEAGSGY NPVRGDKVIA YARRFLDGAV 190 200 210 220 230 240 PLASGSWSDI TGLKLDEGQL AATLDDGGTV GLGTPEQFVG YLGDAEAPTA VLLVNNGLHI 250 260 270 280 290 300 EILIDAEAPI GATDKAGIKD VVLESAITTI MDFEDSVAAV DADDKVLGYR NWLGLNRGDL 310 320 330 340 350 360 AEEVSKGGKT FTRVLNSDRT YTAANPGPDG ATELTLPGRS LLFVRNVGHL MTNDAIVDAQ 370 380 390 400 410 420 GNEIPEGIQD ALFTSLIGIH GLRTGDGNGP LVNSRTGSIY IVKPKMHGPD EVAFTCELFS 430 440 450 460 470 480 RVEDVLGLPQ NTLKVGIMDE ERRTTLNLKA CIKAAADRVV FINTGFLDRT GDEIHTSMDA 490 500 510 520 530 540 GPMIRKGAMK STEWIAAYEN QNVDIGLETG FSGRAQIGKG MWAMTDLMAD MVEQKIGQPR 550 560 570 580 590 600 AGATTAWVPS PTAATLHAMH YHEVDVFAVH NELAGTRRGT VDQLLTIPLA KELAWAPEEI 610 620 630 640 650 660 REEVDNNCQS ILGYVVRWID AGVGCSKVPD IHDVALMEDR ATLRISSQLL ANWLRHGVIT 670 680 690 700 710 720 EEDVKASLRR MAAVVDEQNA ADPDFRPMAP DPDGSIAFQA AQELILSGAD QPNGYTEPIL 730 HRRRREFKAA SNVG

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000511 Genomic DNA. Translation: ABM13899.1.
RefSeq	YP_953905.1.
3D structure databases
SMR	A1T9P9. Positions 2-729.
ModBase	Search...
Protein-protein interaction databases
STRING	A1T9P9.
Genome annotation databases
GeneID	4646853.
GenomeReviews	Gene locus Mvan_3097 in contig CP000511_GR.
KEGG	mva:Mvan_3097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKMHGPD.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCVP

Accession

Primary (citable) accession number: A1T9P9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)