A1T9L9 (MDH_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Mvan_3067

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity. HAMAP MF_01517
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Malate dehydrogenase HAMAP MF_01517		PRO_0000292373
Regions
	Nucleotide binding	15 – 21	7	NAD By similarity
	Nucleotide binding	133 – 135	3	NAD By similarity
Sites
	Active site	191	1	Proton acceptor By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	109	1	NAD By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1T9L9 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 318F708372599FB7	FASTA	332	34,506
	10 20 30 40 50 60 MSSTTASPVT VAVTGAAGHI GYAALFRIAA GAMLGHTTPV TLRLLELPDA VRAAEGVVME 70 80 90 100 110 120 LEDGAFPLLA GTEIYDDPTR AFDGVDVALL IGARPRTKGM ERADLLGANA EIFATSGKAL 130 140 150 160 170 180 NAGASPDVRV LVVGNPANTN ALVASAHAPD IPADRFTALT RLDHNRAVAA TARHSGVPVT 190 200 210 220 230 240 EISRMTIWGN HSPTQYPDIF HAVVGGRSGA DYAADTRWLT DDFIPTVARR GTAIIEARGA 250 260 270 280 290 300 SSAASAANGA IDHIDDWVHG TPDGDWTSVA LPSPGAYGVD EGLVCSFPCR SVGGRWEIVE 310 320 330 GLDINPFSRA RIDASVAELR TERDAVAALG LL

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM13869.1.
RefSeq	YP_953875.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1T9L9.
SMR	A1T9L9. Positions 6-332.
ModBase	Search...
Protein-protein interaction databases
STRING	A1T9L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000083385; EBMYCP00000081338; EBMYCG00000083380.
GeneID	4646973.
GenomeReviews	Gene locus Mvan_3067 in contig CP000511_GR.
KEGG	mva:Mvan_3067.
PATRIC	18184407. VBIMycVan31953_3125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
GeneTree	EBGT00050000018111.
HOGENOM	HBG289884.
OMA	NKIQISL.
ProtClustDB	PRK05442.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_3067-MONOMER.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_MYCVP

Accession

Primary (citable) accession number: A1T9L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program