A1T8U1 (A1T8U1_MYCVP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Mvan_2784

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL ABM13591.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	246	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	283	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	379	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A1T8U1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3B4CA73D6BAF528A	FASTA	576	63,676
	10 20 30 40 50 60 MPEHEFAVWA PRPERVRLDV DGTLHPMTRG DDDWWRATVD APADARYGFV LDDDPKVLPD 70 80 90 100 110 120 PRSPRQPDGV HERSQLWRPQ ADPWTDSGWQ GRSVEGAVVY ELHVGTFTPG GTFDMVVDKL 130 140 150 160 170 180 DHLVGLGVDF VEVMPVNAFG GTHGWGYDGV LWYAVHEPYG GPDGLIRLVD ACHARGLGVL 190 200 210 220 230 240 IDAVFNHLGP SGNYLPKFGP YLSSGSNPWG ESINIGDAGA DEVRRYILDC ALRWMRDFHA 250 260 270 280 290 300 DGLRLDAVHA LVDTTAIHIL EELSAETDAL ADELGRPLSL IAESDMNDPR LITPREHGGL 310 320 330 340 350 360 GMAAQWDDDI HHAIHTAVSG ERQGYYGDFG TLETLSETLR HGYFHAGSYS SFRRRRHGRP 370 380 390 400 410 420 LDTAAVPATR LLAYTLTHDQ VGNRAVGDRP SQNLTTGQLA VKAALALGSP YTAMLFMGEE 430 440 450 460 470 480 WASSSPFQFF SSHPEPELAR ATAEGRKREF AEHGWDADEI PDPQDPETFE RSKLQWDEVG 490 500 510 520 530 540 VGDHARLLEF YRSLIALRHT EPDMADPWLD HLKIDFDEHA RWFVMHRGAL AIACNLGADP 550 560 570 VDVPVTGDVV LAWDEPTVGV DTTRVGGHSV AILRAA

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM13591.1.
RefSeq	YP_953597.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1T8U1.
ModBase	Search...
Protein-protein interaction databases
STRING	A1T8U1.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000079839; EBMYCP00000077792; EBMYCG00000079834.
GeneID	4645313.
GenomeReviews	Gene locus Mvan_2784 in contig CP000511_GR.
KEGG	mva:Mvan_2784.
PATRIC	18183837. VBIMycVan31953_2841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
GeneTree	EBGT00050000016657.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK799816.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1T8U1_MYCVP

Accession

Primary (citable) accession number: A1T8U1

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)