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A1T8K1 (RIBBA_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Riboflavin biosynthesis protein ribBA

Including the following 2 domains:

  1. 3,4-dihydroxy-2-butanone 4-phosphate synthase
    Short name=DHBP synthase
    EC=4.1.99.12
  2. GTP cyclohydrolase-2
    EC=3.5.4.25
    Alternative name(s):
    GTP cyclohydrolase II
Gene names
Name:ribBA
Ordered Locus Names:Mvan_2694
OrganismMycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]
Taxonomic identifier350058 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_01283

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP MF_01283

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. HAMAP MF_01283

Cofactor

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01283

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283

Sequence similarities

In the N-terminal section; belongs to the DHBP synthase family.

In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Riboflavin biosynthesis protein ribBA HAMAP MF_01283
PRO_1000067428

Regions

Nucleotide binding259 – 2635GTP By similarity
Nucleotide binding303 – 3053GTP By similarity
Region1 – 204204DHBP synthase HAMAP MF_01283
Region28 – 292D-ribulose 5-phosphate binding By similarity
Region141 – 1455D-ribulose 5-phosphate binding By similarity
Region205 – 423219GTP cyclohydrolase II HAMAP MF_01283

Sites

Active site3371Proton acceptor; for GTP cyclohydrolase activity Potential
Active site3391Nucleophile; for GTP cyclohydrolase activity By similarity
Metal binding291Magnesium or manganese 1 By similarity
Metal binding291Magnesium or manganese 2 By similarity
Metal binding1441Magnesium or manganese 2 By similarity
Metal binding2641Zinc; catalytic By similarity
Metal binding2751Zinc; catalytic By similarity
Metal binding2771Zinc; catalytic By similarity
Binding site331D-ribulose 5-phosphate By similarity
Binding site1651D-ribulose 5-phosphate By similarity
Binding site2801GTP By similarity
Binding site3251GTP By similarity
Binding site3601GTP By similarity
Binding site3651GTP By similarity
Site1271Essential for DHBP synthase activity By similarity
Site1651Essential for DHBP synthase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A1T8K1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: AA8A617E7BCBD654

FASTA42345,747
        10         20         30         40         50         60 
MTRLDSVERA VADIAAGKAV VVIDDEDREN EGDLIFAAEK ATPELVAFMV RYTSGYLCVP 

        70         80         90        100        110        120 
LAGDICDRLG LLPMYAVNQD KHGTAYTVTV DAKKGVGTGI SAMDRATTMR LLADPDAVAD 

       130        140        150        160        170        180 
DFTKPGHVVP LRAKDGGVLR RPGHTEAAVD LARLAGLQPA GAICEIVSQK DEGAMAQTDE 

       190        200        210        220        230        240 
LRIFADEHDL ALISIADLIE WRRKHEKHIA RVAEARIPTR HGEFRAVGYT SIYEDVEHVA 

       250        260        270        280        290        300 
LVKGEISGPH SDGHDVLVRV HSECLTGDVF GSRRCDCGPQ LDAALAMVAR EGRGVVLYMR 

       310        320        330        340        350        360 
GHEGRGIGLM HKLQAYQLQD AGDDTVDANL KLGLPADARD YGTGAQILVD LGVRSMRLLT 

       370        380        390        400        410        420 
NNPAKRVGLD GYGLHIIERV PLPVRANAEN IRYLMTKRDR MGHDLVGLED FDEAVPGELG 


GAV

« Hide

References

[1]"Complete sequence of Mycobacterium vanbaalenii PYR-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000511 Genomic DNA. Translation: ABM13501.1.
RefSeqYP_953507.1. NC_008726.1.

3D structure databases

ProteinModelPortalA1T8K1.
SMRA1T8K1. Positions 3-204, 208-384.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1T8K1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000080722; EBMYCP00000078675; EBMYCG00000080717.
GeneID4645449.
GenomeReviewsGene locus Mvan_2694 in contig CP000511_GR.
KEGGmva:Mvan_2694.
PATRIC18183655. VBIMycVan31953_2750.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0807.
GeneTreeEBGT00050000015301.
HOGENOMHBG735778.
OMARCDCRMQ.
PhylomeDBA1T8K1.
ProtClustDBPRK09311.

Enzyme and pathway databases

BioCycMVAN350058:MVAN_2694-MONOMER.

Family and domain databases

HAMAPMF_01283. RibBA.
[Tree]
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
Gene3DG3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KOK14652.
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
SUPFAMSSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMsTIGR00505. RibA. 1 hit.
TIGR00506. RibB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRIBBA_MYCVP
AccessionPrimary (citable) accession number: A1T8K1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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