A1T882 (RUVC_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	Mvan_2573

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium vanbaalenii

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the RuvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_1000002782
Sites
	Metal binding	7	1	Magnesium By similarity
	Metal binding	68	1	Magnesium By similarity
	Metal binding	141	1	Magnesium By similarity
	Metal binding	144	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1T882 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 9BAD9EE44A111451	FASTA	194	20,774
	10 20 30 40 50 60 MRVMGVDPGL TRCGLSVIES GKGRQVIALD VDVVRTPAGH PLAHRLLAIS DAVDHWLDTH 70 80 90 100 110 120 RPDVIAIERV FSNQNANTAM GTAQAGGVIA LAAARRDIDV HFHTPSEVKA AVTGNGRADK 130 140 150 160 170 180 AQVTEMVTRI LALQQKPTPA DAADALALAI CHCWRAPMLG RMAAAEEMAA EQRRKYQATL 190 KAKAKAAQMS RSTR

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM13382.1.
RefSeq	YP_953388.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1T882.
ModBase	Search...
Protein-protein interaction databases
STRING	350058.Mvan_2573.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABM13382; ABM13382; Mvan_2573.
GeneID	4643818.
KEGG	mva:Mvan_2573.
PATRIC	18183413. VBIMycVan31953_2629.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0817.
HOGENOM	HOG000012182.
KO	K01159.
OMA	YTALQMK.
ProtClustDB	PRK00039.
Enzyme and pathway databases
BioCyc	MVAN350058:GIWR-2591-MONOMER.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_MYCVP

Accession

Primary (citable) accession number: A1T882

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program