A1T7H2 (SYP_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mvan_2308

Organism

Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]

Taxonomic identifier

350058 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Proline--tRNA ligase HAMAP MF_01569		PRO_0000288354

Sequences

	Sequence		Length	Mass (Da)
	A1T7H2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: FF8EE0F5032E7C94	FASTA	585	63,828
	10 20 30 40 50 60 MITRMSELFL RTLRDDPADA EVPSHKLLIR AGYVRPVGPG LYSWLPLGLK VLRKIENIVR 70 80 90 100 110 120 NEMNAIGGQE ILFPALLPRA PYETTNRWTE YGDTLFRLQD RRNNDYLLGP THEEMFTLTV 130 140 150 160 170 180 KGEYSSYKDF PLRLYQIQNK YRDEARPRAG ILRGREFLMK DSYSFDIDDD GLKNAYIAHR 190 200 210 220 230 240 EAYQRIFDLL KVRYVIVSAV SGAMGGSASE EFLAESDVGE DTYVRCLQSG YAANVEAVVT 250 260 270 280 290 300 MVPEPISSEQ IAGLASATVH HTGDTPTIAT LVDWANGADL GRTVTAADTL KNVMLKVREP 310 320 330 340 350 360 GGEWELLGVG VPGDREVDDK RLGAALEPAE YAMLDDADFA RYPFLVKGYI GPKALLANGV 370 380 390 400 410 420 RYLVDPRVVD GTAWITGADE LGKHVVDLVA GRDFTPDGTI EAAEVREGDP SPDGAGPLVA 430 440 450 460 470 480 ARGIEIGHVF QLGRKYADAF GADVLGENGK PVRLTMGSYG IGVSRLVAVI AEQHHDELGL 490 500 510 520 530 540 RWPSTVSPFD VHVVIANKDA DARTGATELA AELDRLGVDV LLDDRTASPG VKFKDAELLG 550 560 570 580 VPWIVVVGRG WADGIVELRD RFGGDKHEIP VDGAAERISA ALAGA

References

[1]

"Complete sequence of Mycobacterium vanbaalenii PYR-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000511 Genomic DNA. Translation: ABM13122.1.
RefSeq	YP_953128.1. NC_008726.1.
3D structure databases
ProteinModelPortal	A1T7H2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1T7H2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000077663; EBMYCP00000075616; EBMYCG00000077658.
GeneID	4644343.
GenomeReviews	Gene locus Mvan_2308 in contig CP000511_GR.
KEGG	mva:Mvan_2308.
PATRIC	18182863. VBIMycVan31953_2358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
GeneTree	EBGT00050000016802.
HOGENOM	HBG403504.
OMA	IQPAELW.
PhylomeDB	A1T7H2.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	MVAN350058:MVAN_2308-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_MYCVP

Accession

Primary (citable) accession number: A1T7H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program