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A1T3R3

- MEND_MYCVP

UniProt

A1T3R3 - MEND_MYCVP

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMVAN350058:GIWR-980-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:Mvan_0975
OrganismiMycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Taxonomic identifieri350058 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000009159: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5485482-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341788Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi350058.Mvan_0975.

Structurei

3D structure databases

ProteinModelPortaliA1T3R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

A1T3R3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPSTAQARV VVDELIRGGV RDVVLCPGSR NAPLAFALHD ADRAGRLRLH
60 70 80 90 100
VRIDERTAGF LAIGLAVAER APVCVAMTSG TAVANLGPAV VEANYARVPL
110 120 130 140 150
IVLSANRPYE LLGTGANQTF EQLGYFGTQV RANISLGLAP ESGASALNFS
160 170 180 190 200
ALNGQWRSAT CRVVVAATGA RSANAGPVQF DIPLREPLVP DAQEPAVPYA
210 220 230 240 250
PDGRPDGRPW TYTPPVTFDQ PLDIDLTPDT VVIAGHGAGV HPNLAHLPTV
260 270 280 290 300
AEPTAPAAQT PLHPLALRMI RPEQVIMLGR PTLHRPVSAL LADPAVPVFA
310 320 330 340 350
LTTGPRWPDV SGNSQATGTR AVTAGAPAQA WLRRCAEANR HAVDAVRGQL
360 370 380 390 400
AAHPLTTGLH VAAAVADALR PGDQLVLGAS NPVRDIALVG FHTADVKVRS
410 420 430 440 450
NRGVAGIDGT VSTAIGAALA HERSGGRTVA LIGDLTFVHD SSGLLIGPTE
460 470 480 490 500
PTPRKLTIVV SNDNGGGIFE LLEQGDPRFS DVSSRIFGTP HDVDVGALCR
510 520 530 540
AYHVESRQIE VGELAGALDE DFDGMRVLEV KADRSSLRAL HASIRANL
Length:548
Mass (Da):57,366
Last modified:July 1, 2008 - v2
Checksum:i0076891DD4286656
GO

Sequence cautioni

The sequence ABM11813.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000511 Genomic DNA. Translation: ABM11813.1. Different initiation.
RefSeqiYP_951819.1. NC_008726.1.

Genome annotation databases

EnsemblBacteriaiABM11813; ABM11813; Mvan_0975.
GeneIDi4647954.
KEGGimva:Mvan_0975.
PATRICi18180143. VBIMycVan31953_1005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000511 Genomic DNA. Translation: ABM11813.1 . Different initiation.
RefSeqi YP_951819.1. NC_008726.1.

3D structure databases

ProteinModelPortali A1T3R3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 350058.Mvan_0975.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM11813 ; ABM11813 ; Mvan_0975 .
GeneIDi 4647954.
KEGGi mva:Mvan_0975.
PATRICi 18180143. VBIMycVan31953_1005.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci MVAN350058:GIWR-980-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 7251 / PYR-1.

Entry informationi

Entry nameiMEND_MYCVP
AccessioniPrimary (citable) accession number: A1T3R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: November 26, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3