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A1T3R3 (MEND_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:Mvan_0975
OrganismMycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]
Taxonomic identifier350058 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence caution

The sequence ABM11813.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5485482-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341788

Sequences

Sequence LengthMass (Da)Tools
A1T3R3 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: 0076891DD4286656

FASTA54857,366
        10         20         30         40         50         60 
MNPSTAQARV VVDELIRGGV RDVVLCPGSR NAPLAFALHD ADRAGRLRLH VRIDERTAGF 

        70         80         90        100        110        120 
LAIGLAVAER APVCVAMTSG TAVANLGPAV VEANYARVPL IVLSANRPYE LLGTGANQTF 

       130        140        150        160        170        180 
EQLGYFGTQV RANISLGLAP ESGASALNFS ALNGQWRSAT CRVVVAATGA RSANAGPVQF 

       190        200        210        220        230        240 
DIPLREPLVP DAQEPAVPYA PDGRPDGRPW TYTPPVTFDQ PLDIDLTPDT VVIAGHGAGV 

       250        260        270        280        290        300 
HPNLAHLPTV AEPTAPAAQT PLHPLALRMI RPEQVIMLGR PTLHRPVSAL LADPAVPVFA 

       310        320        330        340        350        360 
LTTGPRWPDV SGNSQATGTR AVTAGAPAQA WLRRCAEANR HAVDAVRGQL AAHPLTTGLH 

       370        380        390        400        410        420 
VAAAVADALR PGDQLVLGAS NPVRDIALVG FHTADVKVRS NRGVAGIDGT VSTAIGAALA 

       430        440        450        460        470        480 
HERSGGRTVA LIGDLTFVHD SSGLLIGPTE PTPRKLTIVV SNDNGGGIFE LLEQGDPRFS 

       490        500        510        520        530        540 
DVSSRIFGTP HDVDVGALCR AYHVESRQIE VGELAGALDE DFDGMRVLEV KADRSSLRAL 


HASIRANL 

« Hide

References

[1]"Complete sequence of Mycobacterium vanbaalenii PYR-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000511 Genomic DNA. Translation: ABM11813.1. Different initiation.
RefSeqYP_951819.1. NC_008726.1.

3D structure databases

ProteinModelPortalA1T3R3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350058.Mvan_0975.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM11813; ABM11813; Mvan_0975.
GeneID4647954.
KEGGmva:Mvan_0975.
PATRIC18180143. VBIMycVan31953_1005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OrthoDBEOG6NWBQW.
ProtClustDBPRK07449.

Enzyme and pathway databases

BioCycMVAN350058:GIWR-980-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_MYCVP
AccessionPrimary (citable) accession number: A1T3R3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: February 19, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways