mshA

Functionⁱ

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityⁱ

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	26	1D-inositol 3-phosphateUniRule annotation	1
Binding siteⁱ	40	UDP-GlcNAc; via amide nitrogenUniRule annotation	1
Binding siteⁱ	95	1D-inositol 3-phosphateUniRule annotation	1
Binding siteⁱ	128	1D-inositol 3-phosphateUniRule annotation	1
Binding siteⁱ	152	1D-inositol 3-phosphateUniRule annotation	1
Binding siteⁱ	172	1D-inositol 3-phosphateUniRule annotation	1
Binding siteⁱ	246	UDP-GlcNAcUniRule annotation	1
Binding siteⁱ	251	UDP-GlcNAcUniRule annotation	1
Binding siteⁱ	313	UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation	1
Metal bindingⁱ	322	Magnesium; via carbonyl oxygenUniRule annotation	1
Metal bindingⁱ	323	Magnesium; via carbonyl oxygenUniRule annotation	1
Metal bindingⁱ	325	Magnesium; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	335	UDP-GlcNAcUniRule annotation	1
Binding siteⁱ	343	UDP-GlcNAcUniRule annotation	1
Metal bindingⁱ	349	MagnesiumUniRule annotation	1

GO - Molecular functionⁱ

acetylglucosaminyltransferase activity Source: InterPro
metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

mycothiol biosynthetic process Source: InterPro

Complete GO annotation...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Ligand	Magnesium, Metal-binding

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT4. Glycosyltransferase Family 4.

CAZyⁱ

GT4. Glycosyltransferase Family 4.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation) Alternative name(s): N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation Short name: GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesⁱ	Name:mshAUniRule annotation Ordered Locus Names:Mvan_0827
Organismⁱ	Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Taxonomic identifierⁱ	350058 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Actinobacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium vanbaalenii
Proteomesⁱ	UP000009159 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000400145	1 – 450	D-inositol 3-phosphate glycosyltransferaseAdd BLAST		450

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	350058.Mvan_0827.

STRINGⁱ

350058.Mvan_0827.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	A1T3B5.
SMRⁱ	A1T3B5.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	32 – 33	UDP-GlcNAc bindingUniRule annotation		2
Regionⁱ	37 – 42	1D-inositol 3-phosphate bindingUniRule annotation		6

Sequence similaritiesⁱ

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4107R5M. Bacteria. COG0438. LUCA.
HOGENOMⁱ	HOG000077288.
KEGG Orthology (KO) More...KOⁱ	K15521.
OMAⁱ	HTMAKVK.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H0AO3.

Family and domain databases

HAMAPⁱ	MF_01695. MshA. 1 hit.
InterProⁱ	View protein in InterPro IPR001296. Glyco_trans_1. IPR028098. Glyco_trans_4-like_N. IPR017814. Mycothiol_biosynthesis_MshA.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13439. Glyco_transf_4. 1 hit. PF00534. Glycos_transf_1. 1 hit.
TIGRFAMsⁱ	TIGR03449. mycothiol_MshA. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

A1T3B5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRLATEPASL PSGLPEPRRV AVLSVHTSPL AQPGTGDAGG MNVYVLQTAL 
        60         70         80         90        100
ELANRGVDVE IFTRATSSAD QPVVPVAPGV LVRNVVAGPF EGLDKNDLPT 
       110        120        130        140        150
QLCAFTAGVL RAEATHEPGY YDIVHSHYWL SGQVGWLAAD RWAVPLVHTA 
       160        170        180        190        200
HTLAAVKNAS LAAGDAPEPP MRSIGEQQVV DAADRLIVNT EHEAQQLVSL 
       210        220        230        240        250
HQADPARIDV VHPGVDLATF TPGDRLAARA ALGLDANSRI VAFVGRIQPL 
       260        270        280        290        300
KAPDVLLRAA ALLPDVQVLI AGGPSGSGLI SRPGAGGLAT PDNLVNLAAE 
       310        320        330        340        350
LGMTDRVTFL PPQSRDNLVQ VYRAADVVAV PSYSESFGLV AVEAQACGTP 
       360        370        380        390        400
VVAAAVGGLP VAVRDGVSGA LVDGHDPGDW AGTLADVLAA DPATLSRAAV 
       410        420        430        440        450
DHAATFSWSH TVDALLAGYG RAIADHRARH QGQLARRSGR RFSMRRGVRA

Length:450

Mass (Da):46,802

Last modified:February 6, 2007 - v1

Checksum:ⁱ22BB995E57C26DA3

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000511 Genomic DNA. Translation: ABM11665.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011778101.1. NC_008726.1.

Genome annotation databases

EnsemblBacteriaⁱ	ABM11665; ABM11665; Mvan_0827.
KEGGⁱ	mva:Mvan_0827.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000511 Genomic DNA. Translation: ABM11665.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011778101.1. NC_008726.1.

3D structure databases

ProteinModelPortalⁱ	A1T3B5.
SMRⁱ	A1T3B5.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	350058.Mvan_0827.

STRINGⁱ

350058.Mvan_0827.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GT4. Glycosyltransferase Family 4.

CAZyⁱ

GT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABM11665; ABM11665; Mvan_0827.
KEGGⁱ	mva:Mvan_0827.

Phylogenomic databases

eggNOGⁱ	ENOG4107R5M. Bacteria. COG0438. LUCA.
HOGENOMⁱ	HOG000077288.
KEGG Orthology (KO) More...KOⁱ	K15521.
OMAⁱ	HTMAKVK.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H0AO3.

Family and domain databases

HAMAPⁱ	MF_01695. MshA. 1 hit.
InterProⁱ	View protein in InterPro IPR001296. Glyco_trans_1. IPR028098. Glyco_trans_4-like_N. IPR017814. Mycothiol_biosynthesis_MshA.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13439. Glyco_transf_4. 1 hit. PF00534. Glycos_transf_1. 1 hit.
TIGRFAMsⁱ	TIGR03449. mycothiol_MshA. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MSHA_MYCVP
Accessionⁱ	A1T3B5Primary (citable) accession number: A1T3B5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
	Last sequence update:	February 6, 2007
	Last modified:	June 7, 2017
	This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A1T3B5 (MSHA_MYCVP)

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D-inositol 3-phosphate glycosyltransferase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ