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A1T1K1 (PCKG_MYCVP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:Mvan_0201
OrganismMycobacterium vanbaalenii (strain DSM 7251 / PYR-1) [Complete proteome] [HAMAP]
Taxonomic identifier350058 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 609609Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000060298

Regions

Nucleotide binding272 – 2776GTP By similarity
Nucleotide binding515 – 5184GTP By similarity
Region387 – 3893Substrate binding By similarity

Sites

Active site2731 By similarity
Metal binding2291Manganese By similarity
Metal binding2491Manganese; via tele nitrogen By similarity
Metal binding2961Manganese By similarity
Binding site811Substrate By similarity
Binding site2221Substrate; via amide nitrogen By similarity
Binding site2291Substrate By similarity
Binding site2711Substrate By similarity
Binding site3891GTP By similarity
Binding site4201GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1T1K1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: B82518B76933A3A6

FASTA60967,664
        10         20         30         40         50         60 
MTAATIPGLD NAPTKHQGLL EWVREVAELT QPDRVAWADG SQEEYDRLCA QLVEAGTFKK 

        70         80         90        100        110        120 
LNPEKQPNSY LALSDPSDVA RVESRTFICS KREIDAGPTN NWMDPAEMRG ILTDLYRGCM 

       130        140        150        160        170        180 
RGRTLWVVPF CMGPLDADDP KLGVEITDSE YVVVSMRTMT RMGAAALQKM GDDGFFVKAL 

       190        200        210        220        230        240 
HSIGAPLEPG QKDVPWPCND TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA 

       250        260        270        280        290        300 
HDEGWLAEHM LILKLISPEN KAYYIAAAFP SACGKTNLAM LQPTIPGWRA ETVGDDIAWM 

       310        320        330        340        350        360 
RFGKDGRLYA TNPEFGFFGV APGTNWDSNP NAMKTIAAGN TVFTNVALTD DHDVWWEGLE 

       370        380        390        400        410        420 
GEPDHLIDWK GQDWILRETE TKAAHPNSRY CTPISQCPTL APEWDDPQGV PISAILFGGR 

       430        440        450        460        470        480 
RKTTVPLITE ARDWQHGVFI GATLGSEQTA AAEGKVGTVR RDPMAMLPFL GYNVGDYFQH 

       490        500        510        520        530        540 
WINIGKQADE SQLPKVFFVN WFRRGDDGRF LWPGFGENSR VLKWAIERIE HKADGKSTPI 

       550        560        570        580        590        600 
GIVPTAADLD LEGLDVDPAD VDEALAVNAD EWRAEVPLIE EWFEFVGEKL PTGIKDEFDA 


LKTRLAEDS 

« Hide

References

[1]"Complete sequence of Mycobacterium vanbaalenii PYR-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.J., Miller C., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 7251 / PYR-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000511 Genomic DNA. Translation: ABM11051.1.
RefSeqYP_951057.1. NC_008726.1.

3D structure databases

ProteinModelPortalA1T1K1.
SMRA1T1K1. Positions 12-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350058.Mvan_0201.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM11051; ABM11051; Mvan_0201.
GeneID4647839.
KEGGmva:Mvan_0201.
PATRIC18178536. VBIMycVan31953_0204.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.
OrthoDBEOG6MPWQS.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycMVAN350058:GIWR-204-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_MYCVP
AccessionPrimary (citable) accession number: A1T1K1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways