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A1SZP3

- SYI_PSYIN

UniProt

A1SZP3 - SYI_PSYIN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Psychromonas ingrahamii (strain 37)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi927 – 9271ZincUniRule annotation
    Metal bindingi930 – 9301ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPING357804:GJBJ-3380-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Ping_3271
    OrganismiPsychromonas ingrahamii (strain 37)
    Taxonomic identifieri357804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
    ProteomesiUP000000639: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 944944Isoleucine--tRNA ligasePRO_1000022110Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi357804.Ping_3271.

    Structurei

    3D structure databases

    ProteinModelPortaliA1SZP3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1SZP3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKSTLNL PKTGFPMRAN LANREPNMLK NWYSNDLYGK IRAAKKGKKT    50
    FILHDGPPYA NGDIHIGHSV NKILKDIIIK SKTLSDFDAP YIPGWDCHGL 100
    PIELKVEQKV GKPGKKISAA EFRKKCREYA ARQVDGQRKD FKRLGVLGEW 150
    DKPYLTMDFG TEANIVRALA QVIKNDHLHK GSKPVHWCTE CGSALAEAEV 200
    EYEDKLSPAI DVAFSAVDNE QVLNCFSAVT DNLGEGNVSA VIWTTTPWTL 250
    PANRAIALAQ KVEYSLVQAG ERRLIIATDL VADCLKRYGN EDFSTLAICK 300
    GIDLEKQLFN HPFLDLQVPA ILADYVTVDA GTGCVHTAPG HGQDDYAVGL 350
    RYDLEVANYV ADNGVFRDDT EFFAGLHVFK ANDRVLEVLA ERNALLCSIK 400
    LNHSYPHCWR HKTPIIFRAT PQWFIAMDQK SLRADALAEI QAVKWIPSWG 450
    QQRIEKMVEN RPDWCISRQR TWGVPITLFV HKETDELHPN SVEMMELIAR 500
    KIEIDGIQAW WDLEPETLLG FEAEEYRKVT DTLDVWFDSG TTHASVVAKR 550
    EEYTLESGEQ AAADMYLEGS DQHRGWFQSS LMTSMAINNK APYKEVLTHG 600
    FVVDGNGKKM SKSLGNVMSP QTVMNNLGAD ILRLWVASTD YTGEMTVSDE 650
    ILNRSADSYR RIRNTSRFLL ANLNGFDPTT DLVPNEEMVA LDRWIVAQTL 700
    ILQNDVIEAY ESYSLHAVYQ KLTHFCSIEL GSFYLDIIKD RQYTAKADSN 750
    AHRSCQTALF HIAEALVRLM APILSFTADE IWARLPGARD EFVFTTVWYD 800
    GLFSLSDNEA LSSAAWSKLT LVRAEVNKAL EIARKENAIG GGLEAEVILY 850
    ASDEIATLLT TLEDELRFVL ITSQAQVKPL ADAPQLALKT EVTGLLVSVV 900
    KSAAPKCERC WHHREEVGQN KQHPELCGRC VTNIEGQGEI RKFA 944
    Length:944
    Mass (Da):106,001
    Last modified:February 6, 2007 - v1
    Checksum:i0A3CAA34C1F67913
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000510 Genomic DNA. Translation: ABM04958.1.
    RefSeqiWP_011771510.1. NC_008709.1.
    YP_944557.1. NC_008709.1.

    Genome annotation databases

    EnsemblBacteriaiABM04958; ABM04958; Ping_3271.
    GeneIDi4626304.
    KEGGipin:Ping_3271.
    PATRICi23072071. VBIPsyIng103130_3614.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000510 Genomic DNA. Translation: ABM04958.1 .
    RefSeqi WP_011771510.1. NC_008709.1.
    YP_944557.1. NC_008709.1.

    3D structure databases

    ProteinModelPortali A1SZP3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 357804.Ping_3271.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM04958 ; ABM04958 ; Ping_3271 .
    GeneIDi 4626304.
    KEGGi pin:Ping_3271.
    PATRICi 23072071. VBIPsyIng103130_3614.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PING357804:GJBJ-3380-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 37.

    Entry informationi

    Entry nameiSYI_PSYIN
    AccessioniPrimary (citable) accession number: A1SZP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3