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A1SZP3

- SYI_PSYIN

UniProt

A1SZP3 - SYI_PSYIN

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Psychromonas ingrahamii (strain 37)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi927 – 9271ZincUniRule annotation
Metal bindingi930 – 9301ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPING357804:GJBJ-3380-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ping_3271
OrganismiPsychromonas ingrahamii (strain 37)
Taxonomic identifieri357804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
ProteomesiUP000000639: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 944944Isoleucine--tRNA ligasePRO_1000022110Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi357804.Ping_3271.

Structurei

3D structure databases

ProteinModelPortaliA1SZP3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1SZP3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKSTLNL PKTGFPMRAN LANREPNMLK NWYSNDLYGK IRAAKKGKKT
60 70 80 90 100
FILHDGPPYA NGDIHIGHSV NKILKDIIIK SKTLSDFDAP YIPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGKKISAA EFRKKCREYA ARQVDGQRKD FKRLGVLGEW
160 170 180 190 200
DKPYLTMDFG TEANIVRALA QVIKNDHLHK GSKPVHWCTE CGSALAEAEV
210 220 230 240 250
EYEDKLSPAI DVAFSAVDNE QVLNCFSAVT DNLGEGNVSA VIWTTTPWTL
260 270 280 290 300
PANRAIALAQ KVEYSLVQAG ERRLIIATDL VADCLKRYGN EDFSTLAICK
310 320 330 340 350
GIDLEKQLFN HPFLDLQVPA ILADYVTVDA GTGCVHTAPG HGQDDYAVGL
360 370 380 390 400
RYDLEVANYV ADNGVFRDDT EFFAGLHVFK ANDRVLEVLA ERNALLCSIK
410 420 430 440 450
LNHSYPHCWR HKTPIIFRAT PQWFIAMDQK SLRADALAEI QAVKWIPSWG
460 470 480 490 500
QQRIEKMVEN RPDWCISRQR TWGVPITLFV HKETDELHPN SVEMMELIAR
510 520 530 540 550
KIEIDGIQAW WDLEPETLLG FEAEEYRKVT DTLDVWFDSG TTHASVVAKR
560 570 580 590 600
EEYTLESGEQ AAADMYLEGS DQHRGWFQSS LMTSMAINNK APYKEVLTHG
610 620 630 640 650
FVVDGNGKKM SKSLGNVMSP QTVMNNLGAD ILRLWVASTD YTGEMTVSDE
660 670 680 690 700
ILNRSADSYR RIRNTSRFLL ANLNGFDPTT DLVPNEEMVA LDRWIVAQTL
710 720 730 740 750
ILQNDVIEAY ESYSLHAVYQ KLTHFCSIEL GSFYLDIIKD RQYTAKADSN
760 770 780 790 800
AHRSCQTALF HIAEALVRLM APILSFTADE IWARLPGARD EFVFTTVWYD
810 820 830 840 850
GLFSLSDNEA LSSAAWSKLT LVRAEVNKAL EIARKENAIG GGLEAEVILY
860 870 880 890 900
ASDEIATLLT TLEDELRFVL ITSQAQVKPL ADAPQLALKT EVTGLLVSVV
910 920 930 940
KSAAPKCERC WHHREEVGQN KQHPELCGRC VTNIEGQGEI RKFA
Length:944
Mass (Da):106,001
Last modified:February 6, 2007 - v1
Checksum:i0A3CAA34C1F67913
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM04958.1.
RefSeqiYP_944557.1. NC_008709.1.

Genome annotation databases

EnsemblBacteriaiABM04958; ABM04958; Ping_3271.
GeneIDi4626304.
KEGGipin:Ping_3271.
PATRICi23072071. VBIPsyIng103130_3614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM04958.1 .
RefSeqi YP_944557.1. NC_008709.1.

3D structure databases

ProteinModelPortali A1SZP3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 357804.Ping_3271.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM04958 ; ABM04958 ; Ping_3271 .
GeneIDi 4626304.
KEGGi pin:Ping_3271.
PATRICi 23072071. VBIPsyIng103130_3614.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PING357804:GJBJ-3380-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 37.

Entry informationi

Entry nameiSYI_PSYIN
AccessioniPrimary (citable) accession number: A1SZP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3