Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 3

Gene

topB

Organism
Psychromonas ingrahamii (strain 37)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 1; catalyticUniRule annotation1
Metal bindingi103Magnesium 2UniRule annotation1
Metal bindingi105Magnesium 2UniRule annotation1
Active sitei331O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:Ping_2461Imported
OrganismiPsychromonas ingrahamii (strain 37)Imported
Taxonomic identifieri357804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
Proteomesi
  • UP000000639 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei178Interaction with DNAUniRule annotation1
Sitei333Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi357804.Ping_2461.

Structurei

3D structure databases

ProteinModelPortaliA1SXH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 199Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000086848.
KOiK03169.
OMAiTYPRVDT.
OrthoDBiPOG091H028X.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
cd03362. TOPRIM_TopoIA_TopoIII. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiView protein in InterPro
IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. TOPRIM_domain.
IPR034144. TOPRIM_TopoIII.
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiView protein in Pfam
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PRINTSiPR00417. PRTPISMRASEI.
SMARTiView protein in SMART
SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiView protein in PROSITE
PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.

Sequencei

Sequence statusi: Complete.

A1SXH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLYIAEKPS LARAIADALP KPHRKADGCI YVGNGDVVSW CIGHLLTQVD
60 70 80 90 100
PEAYDPEYKK WKFEHLPIIP ENWKLKSTPR TSKQLSILKK LIKQADNLVH
110 120 130 140 150
VGDPDREGQL LVDEVIHFSG VKGNKLKQVE RCLVSDLTTN AVKRSLQQLR
160 170 180 190 200
SNQEFIALST SALARTRADW LYGLNLTRVY TLQAQKSGYQ GVISVGRVQT
210 220 230 240 250
PLLGLVARRD KEIEVFVSKP FYEVEAHLTT LKPENVAAFT AKWKPSEACE
260 270 280 290 300
KYQDHEGRVL VKKLAENVAQ RISQQHAVVK SFTAKNKKQV APLPYNLSAL
310 320 330 340 350
QIDAAKRFSY TALEVLDICQ ALYEKYKLIT YPRSDSRYLP KNHLSQAGSV
360 370 380 390 400
TRAIANNQPA LKKAVSNADI SLQSRAWNDK KVDAHHAIIP TDKHQSLTSL
410 420 430 440 450
DTASQNIYAL IARQYLCQFY ADYHYGECVA EIEINNGLFI SKAKAKISDG
460 470 480 490 500
WKVLFDKQEK DSYLPALSKG QKLFCDKGEV IEKNTQPPAY FTDATLLAAM
510 520 530 540 550
SNIARYVKDG ELKAVLKETD GLGTEATRAG IIELLFKRLF LQRTGKLIKA
560 570 580 590 600
TETGRKLIES LPEMLTLPDM TAQWEMELNA ISQKEQSYQG FVVPLQNQLK
610 620 630 640
SIIEKAIADG PRALANLPSS GNVFKKTYRK KSTKKGTFKK KGS
Length:643
Mass (Da):72,153
Last modified:February 6, 2007 - v1
Checksum:iF6F166786C07A442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM04192.1.
RefSeqiWP_011770752.1. NC_008709.1.

Genome annotation databases

EnsemblBacteriaiABM04192; ABM04192; Ping_2461.
KEGGipin:Ping_2461.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA1SXH7_PSYIN
AccessioniPrimary (citable) accession number: A1SXH7
Entry historyiIntegrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: June 7, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported