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A1SWQ0

- NAPA_PSYIN

UniProt

A1SWQ0 - NAPA_PSYIN

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Psychromonas ingrahamii (strain 37)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciPING357804:GJBJ-2260-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Ping_2174
    OrganismiPsychromonas ingrahamii (strain 37)
    Taxonomic identifieri357804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
    ProteomesiUP000000639: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 831802Periplasmic nitrate reductasePRO_5000206772Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi357804.Ping_2174.

    Structurei

    3D structure databases

    ProteinModelPortaliA1SWQ0.
    SMRiA1SWQ0. Positions 41-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiRRLSHKD.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1SWQ0-1 [UniParc]FASTAAdd to Basket

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    MKFTRREFMK AQAAASAAAV AGIALPATAS NLIASQDLTK IKWEKAPCRF    50
    CGTGCSVLVG TQHGRVVATQ GDPESPVNKG LNCVKGYFLS KIMYGKDRLT 100
    TPLLRMTEGV YDKNGQFEPV SWDAAFDIMA DKFKDALKKK GPTAVGMFGS 150
    GQWTLWEGYA ASKLMKAGFR TNNLDPNARH CMASAVGGFM RTFGIDEPMG 200
    CYDDLENADV FVLWGSNMAE MHPILWSRLA DRRLSNPDVS VHVLSTYTHR 250
    SFELADNGMI FTPQSDLAIL NFIANYIIQN DAVDKDFIEK HTNFRKGVTD 300
    IGYGLRPTDP LQKAAENPDS GASSPITFEE FAEYVSEFDV DYAVKMSGVP 350
    AEKLIELAKV YADPAKKVVS YWTMGFNQHT RGVWANNLMY NVHLLTGKIS 400
    KPGSGPFSLT GQPSACGTAR EVGTFSHRLP ADLVVANPKH RAIAEKLWKL 450
    PEGTIEPKPG YHAVLQNRML KDSKLNAYWV MCNNNVQAGA NINEEIIPGY 500
    RNPENFIVVS DPYPTVTAQM GDLILPTAMW VEKEGAYGNA ERRTQFWHQQ 550
    VKAPEGAKSD LWQLVEFSKR FKIEEVWTED LIAKMPEVRG ETLFDVLYKN 600
    GQVDAFGLDE VADERLNDEA RDFGFYIQKG LFEEYASFGR GHGHDLAPFD 650
    QYHEARGLRW PVVNGKETLW RFREGYDPYV EKGSEVQFYG HPDKKAVIFA 700
    LPYEPPAESP DQEYDLWLST GRVLEHWHSG SMTRRVPELY KAFPDAVVFM 750
    HPDDATARGV RRGDEIVLAS RRGELTSRVE TRGRNRPPRG LVFMPWFDAK 800
    QLVNKITLDA TDPLSKQTDY KKCAVKITKK V 831
    Length:831
    Mass (Da):93,213
    Last modified:February 6, 2007 - v1
    Checksum:i504DD2EF62D524E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000510 Genomic DNA. Translation: ABM03915.1.
    RefSeqiYP_943514.1. NC_008709.1.

    Genome annotation databases

    EnsemblBacteriaiABM03915; ABM03915; Ping_2174.
    GeneIDi4625329.
    KEGGipin:Ping_2174.
    PATRICi23069605. VBIPsyIng103130_2404.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000510 Genomic DNA. Translation: ABM03915.1 .
    RefSeqi YP_943514.1. NC_008709.1.

    3D structure databases

    ProteinModelPortali A1SWQ0.
    SMRi A1SWQ0. Positions 41-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 357804.Ping_2174.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM03915 ; ABM03915 ; Ping_2174 .
    GeneIDi 4625329.
    KEGGi pin:Ping_2174.
    PATRICi 23069605. VBIPsyIng103130_2404.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi RRLSHKD.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci PING357804:GJBJ-2260-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 37.

    Entry informationi

    Entry nameiNAPA_PSYIN
    AccessioniPrimary (citable) accession number: A1SWQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3