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A1SWQ0 (NAPA_PSYIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Ping_2174
OrganismPsychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]
Taxonomic identifier357804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 831802Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000206772

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SWQ0 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 504DD2EF62D524E5

FASTA83193,213
        10         20         30         40         50         60 
MKFTRREFMK AQAAASAAAV AGIALPATAS NLIASQDLTK IKWEKAPCRF CGTGCSVLVG 

        70         80         90        100        110        120 
TQHGRVVATQ GDPESPVNKG LNCVKGYFLS KIMYGKDRLT TPLLRMTEGV YDKNGQFEPV 

       130        140        150        160        170        180 
SWDAAFDIMA DKFKDALKKK GPTAVGMFGS GQWTLWEGYA ASKLMKAGFR TNNLDPNARH 

       190        200        210        220        230        240 
CMASAVGGFM RTFGIDEPMG CYDDLENADV FVLWGSNMAE MHPILWSRLA DRRLSNPDVS 

       250        260        270        280        290        300 
VHVLSTYTHR SFELADNGMI FTPQSDLAIL NFIANYIIQN DAVDKDFIEK HTNFRKGVTD 

       310        320        330        340        350        360 
IGYGLRPTDP LQKAAENPDS GASSPITFEE FAEYVSEFDV DYAVKMSGVP AEKLIELAKV 

       370        380        390        400        410        420 
YADPAKKVVS YWTMGFNQHT RGVWANNLMY NVHLLTGKIS KPGSGPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFSHRLP ADLVVANPKH RAIAEKLWKL PEGTIEPKPG YHAVLQNRML KDSKLNAYWV 

       490        500        510        520        530        540 
MCNNNVQAGA NINEEIIPGY RNPENFIVVS DPYPTVTAQM GDLILPTAMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTQFWHQQ VKAPEGAKSD LWQLVEFSKR FKIEEVWTED LIAKMPEVRG ETLFDVLYKN 

       610        620        630        640        650        660 
GQVDAFGLDE VADERLNDEA RDFGFYIQKG LFEEYASFGR GHGHDLAPFD QYHEARGLRW 

       670        680        690        700        710        720 
PVVNGKETLW RFREGYDPYV EKGSEVQFYG HPDKKAVIFA LPYEPPAESP DQEYDLWLST 

       730        740        750        760        770        780 
GRVLEHWHSG SMTRRVPELY KAFPDAVVFM HPDDATARGV RRGDEIVLAS RRGELTSRVE 

       790        800        810        820        830 
TRGRNRPPRG LVFMPWFDAK QLVNKITLDA TDPLSKQTDY KKCAVKITKK V 

« Hide

References

[1]"Complete sequence of Psychromonas ingrahamii 37."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 37.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000510 Genomic DNA. Translation: ABM03915.1.
RefSeqYP_943514.1. NC_008709.1.

3D structure databases

ProteinModelPortalA1SWQ0.
SMRA1SWQ0. Positions 41-829.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357804.Ping_2174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM03915; ABM03915; Ping_2174.
GeneID4625329.
KEGGpin:Ping_2174.
PATRIC23069605. VBIPsyIng103130_2404.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRTHVWH.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycPING357804:GJBJ-2260-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PSYIN
AccessionPrimary (citable) accession number: A1SWQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families