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A1SWQ0

- NAPA_PSYIN

UniProt

A1SWQ0 - NAPA_PSYIN

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Psychromonas ingrahamii (strain 37)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPING357804:GJBJ-2260-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Ping_2174
OrganismiPsychromonas ingrahamii (strain 37)
Taxonomic identifieri357804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
ProteomesiUP000000639: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 831802Periplasmic nitrate reductasePRO_5000206772Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi357804.Ping_2174.

Structurei

3D structure databases

ProteinModelPortaliA1SWQ0.
SMRiA1SWQ0. Positions 41-829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiRRLSHKD.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1SWQ0-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKFTRREFMK AQAAASAAAV AGIALPATAS NLIASQDLTK IKWEKAPCRF
60 70 80 90 100
CGTGCSVLVG TQHGRVVATQ GDPESPVNKG LNCVKGYFLS KIMYGKDRLT
110 120 130 140 150
TPLLRMTEGV YDKNGQFEPV SWDAAFDIMA DKFKDALKKK GPTAVGMFGS
160 170 180 190 200
GQWTLWEGYA ASKLMKAGFR TNNLDPNARH CMASAVGGFM RTFGIDEPMG
210 220 230 240 250
CYDDLENADV FVLWGSNMAE MHPILWSRLA DRRLSNPDVS VHVLSTYTHR
260 270 280 290 300
SFELADNGMI FTPQSDLAIL NFIANYIIQN DAVDKDFIEK HTNFRKGVTD
310 320 330 340 350
IGYGLRPTDP LQKAAENPDS GASSPITFEE FAEYVSEFDV DYAVKMSGVP
360 370 380 390 400
AEKLIELAKV YADPAKKVVS YWTMGFNQHT RGVWANNLMY NVHLLTGKIS
410 420 430 440 450
KPGSGPFSLT GQPSACGTAR EVGTFSHRLP ADLVVANPKH RAIAEKLWKL
460 470 480 490 500
PEGTIEPKPG YHAVLQNRML KDSKLNAYWV MCNNNVQAGA NINEEIIPGY
510 520 530 540 550
RNPENFIVVS DPYPTVTAQM GDLILPTAMW VEKEGAYGNA ERRTQFWHQQ
560 570 580 590 600
VKAPEGAKSD LWQLVEFSKR FKIEEVWTED LIAKMPEVRG ETLFDVLYKN
610 620 630 640 650
GQVDAFGLDE VADERLNDEA RDFGFYIQKG LFEEYASFGR GHGHDLAPFD
660 670 680 690 700
QYHEARGLRW PVVNGKETLW RFREGYDPYV EKGSEVQFYG HPDKKAVIFA
710 720 730 740 750
LPYEPPAESP DQEYDLWLST GRVLEHWHSG SMTRRVPELY KAFPDAVVFM
760 770 780 790 800
HPDDATARGV RRGDEIVLAS RRGELTSRVE TRGRNRPPRG LVFMPWFDAK
810 820 830
QLVNKITLDA TDPLSKQTDY KKCAVKITKK V
Length:831
Mass (Da):93,213
Last modified:February 6, 2007 - v1
Checksum:i504DD2EF62D524E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM03915.1.
RefSeqiWP_011770475.1. NC_008709.1.
YP_943514.1. NC_008709.1.

Genome annotation databases

EnsemblBacteriaiABM03915; ABM03915; Ping_2174.
GeneIDi4625329.
KEGGipin:Ping_2174.
PATRICi23069605. VBIPsyIng103130_2404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM03915.1 .
RefSeqi WP_011770475.1. NC_008709.1.
YP_943514.1. NC_008709.1.

3D structure databases

ProteinModelPortali A1SWQ0.
SMRi A1SWQ0. Positions 41-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 357804.Ping_2174.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM03915 ; ABM03915 ; Ping_2174 .
GeneIDi 4625329.
KEGGi pin:Ping_2174.
PATRICi 23069605. VBIPsyIng103130_2404.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi RRLSHKD.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci PING357804:GJBJ-2260-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 37.

Entry informationi

Entry nameiNAPA_PSYIN
AccessioniPrimary (citable) accession number: A1SWQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3