Reviewed, UniProtKB/Swiss-Prot A1SWB8 (BGAL_PSYIN)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Lactase

Gene names

Name:	lacZ
Ordered Locus Names:	Ping_2019

Organism

Psychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]

Taxonomic identifier

357804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Psychromonadaceae › Psychromonas

Protein attributes

Sequence length	1035 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687
Cofactor	Binds 2 magnesium ions per monomer By similarity. Binds 1 sodium ion per monomer By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Sodium
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: HAMAP sodium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1035	1035	Beta-galactosidase HAMAP MF_01687		PRO_0000367006
Regions
	Region	540 – 543	4	Substrate binding By similarity
Sites
	Active site	460	1	Proton donor By similarity
	Active site	540	1	Nucleophile By similarity
	Metal binding	199	1	Sodium By similarity
	Metal binding	415	1	Magnesium 1 By similarity
	Metal binding	417	1	Magnesium 1 By similarity
	Metal binding	460	1	Magnesium 1 By similarity
	Metal binding	600	1	Magnesium 2 By similarity
	Metal binding	604	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	607	1	Sodium By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	199	1	Substrate By similarity
	Binding site	460	1	Substrate By similarity
	Binding site	607	1	Substrate By similarity
	Binding site	1011	1	Substrate By similarity
	Site	356	1	Transition state stabilizer By similarity
	Site	390	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SWB8-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: EFBFDF2F17314885	FASTA	1,035	117,460
	10 20 30 40 50 60 MREFSEIIKA RDWENQEVTH QHVVQAHAPL HAFHSKQAAL DNQASEFQQL LNGQWRFQLF 70 80 90 100 110 120 AKPEAVPNHC INIDFDDSAW SDITVPSNWQ LQGYDKPIYT NVKYPFADNP PFVPADNPTG 130 140 150 160 170 180 VYRLNFTLPT AWKERKNTVI FDGVNSAFHL WCNGIWVGYS QDSRLPAEFD LSCHLQAGDN 190 200 210 220 230 240 QLTVMVLRWS DGSYLEDQDM WWLSGIFRDV CLLSKPLISI RDITVSTELD ACFNHGSINV 250 260 270 280 290 300 VTQLSEQSSQ YTAQVQLFDA QLQPVTKLVG APFGERMIDE KGPANDRAEH KIAVPSPHKW 310 320 330 340 350 360 SSESPYLYRV VISLVDNEGQ VVDSEAYQVG FRVVEMSNGQ LKLNGEALLI RGVNRHEHHP 370 380 390 400 410 420 EKGHAISYED MLVDIKLLKQ NNFNAVRTAH YPNHPLWYEL CDQYGLYVVD EANLETHGQF 430 440 450 460 470 480 PMSRLSNDLS WLNAYMRRMT RMVERDKNHP SIIIWSLGNE SGLGHHHHAM YQWTKRRDPT 490 500 510 520 530 540 RPVQYEGGGA DTAATDIIVP MYARVNKDIT LPNAPDVVPK MAIKKWLSMP NEQRPLILCE 550 560 570 580 590 600 YAHAMGNSLG SFDKYWQAFR EYPRLQGGFI WDWVDQGLTK IDDNGDNYWA YGGDFGDQIN 610 620 630 640 650 660 DRQFCINGLV FPDRSLHPTV YEAKKAQQFY QFSLVDGDQL KVKIDSENLF IESMDETLCW 670 680 690 700 710 720 SVTEAGYVIA SGEMELHVTA QSSKILTLLE SYPEQKIGCD YFLNIEIVLN KDKPWATKGF 730 740 750 760 770 780 VVATEQVALA SIAQLTNVPL IESGAPRLSE DKNKITVAGT GSGAEFELEI DKQQGVISQW 790 800 810 820 830 840 LVRGENKILQ GPKDNFFRAP LDNDIGTSEA DCIDPNAWVT QWDTAGIANL VPHCIAIEAV 850 860 870 880 890 900 TLARSVLVKV EFGHYVENKL LISSHWQYTI NNQGEVQIDV NVNLAKSLSP LPRIGLELIL 910 920 930 940 950 960 PDSEKPVNWF GRGPHENYPD RILSAHIARH CCSIEEMHTP YIFPSENGLR CDVKDAIVGD 970 980 990 1000 1010 1020 LTVSGDFHLA VSRYSQMNIA QAKHVNDLIN DHQLYVRLDA FHMGVGGDDS WSPSVHDEFL 1030 LNKEHYHYQM ILAFN

References

[1]

"Complete sequence of Psychromonas ingrahamii 37." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000510 Genomic DNA. Translation: ABM03783.1.
RefSeq	YP_943382.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1SWB8.
Genome annotation databases
GeneID	4624702.
GenomeReviews	Gene locus Ping_2019 in contig CP000510_GR.
KEGG	pin:Ping_2019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NELLHWM.
Family and domain databases
HAMAP	MF_01687. [Tree]
InterPro	IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR004199. Glyco_hydro_42_D5. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_PSYIN

Accession

Primary (citable) accession number: A1SWB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)