Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1SWB8 (BGAL_PSYIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:Ping_2019
OrganismPsychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]
Taxonomic identifier357804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10351035Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367006

Regions

Region540 – 5434Substrate binding By similarity

Sites

Active site4601Proton donor By similarity
Active site5401Nucleophile By similarity
Metal binding1991Sodium By similarity
Metal binding4151Magnesium 1 By similarity
Metal binding4171Magnesium 1 By similarity
Metal binding4601Magnesium 1 By similarity
Metal binding6001Magnesium 2 By similarity
Metal binding6041Sodium; via carbonyl oxygen By similarity
Metal binding6071Sodium By similarity
Binding site1011Substrate By similarity
Binding site1991Substrate By similarity
Binding site4601Substrate By similarity
Binding site6071Substrate By similarity
Binding site10111Substrate By similarity
Site3561Transition state stabilizer By similarity
Site3901Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SWB8 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: EFBFDF2F17314885

FASTA1,035117,460
        10         20         30         40         50         60 
MREFSEIIKA RDWENQEVTH QHVVQAHAPL HAFHSKQAAL DNQASEFQQL LNGQWRFQLF 

        70         80         90        100        110        120 
AKPEAVPNHC INIDFDDSAW SDITVPSNWQ LQGYDKPIYT NVKYPFADNP PFVPADNPTG 

       130        140        150        160        170        180 
VYRLNFTLPT AWKERKNTVI FDGVNSAFHL WCNGIWVGYS QDSRLPAEFD LSCHLQAGDN 

       190        200        210        220        230        240 
QLTVMVLRWS DGSYLEDQDM WWLSGIFRDV CLLSKPLISI RDITVSTELD ACFNHGSINV 

       250        260        270        280        290        300 
VTQLSEQSSQ YTAQVQLFDA QLQPVTKLVG APFGERMIDE KGPANDRAEH KIAVPSPHKW 

       310        320        330        340        350        360 
SSESPYLYRV VISLVDNEGQ VVDSEAYQVG FRVVEMSNGQ LKLNGEALLI RGVNRHEHHP 

       370        380        390        400        410        420 
EKGHAISYED MLVDIKLLKQ NNFNAVRTAH YPNHPLWYEL CDQYGLYVVD EANLETHGQF 

       430        440        450        460        470        480 
PMSRLSNDLS WLNAYMRRMT RMVERDKNHP SIIIWSLGNE SGLGHHHHAM YQWTKRRDPT 

       490        500        510        520        530        540 
RPVQYEGGGA DTAATDIIVP MYARVNKDIT LPNAPDVVPK MAIKKWLSMP NEQRPLILCE 

       550        560        570        580        590        600 
YAHAMGNSLG SFDKYWQAFR EYPRLQGGFI WDWVDQGLTK IDDNGDNYWA YGGDFGDQIN 

       610        620        630        640        650        660 
DRQFCINGLV FPDRSLHPTV YEAKKAQQFY QFSLVDGDQL KVKIDSENLF IESMDETLCW 

       670        680        690        700        710        720 
SVTEAGYVIA SGEMELHVTA QSSKILTLLE SYPEQKIGCD YFLNIEIVLN KDKPWATKGF 

       730        740        750        760        770        780 
VVATEQVALA SIAQLTNVPL IESGAPRLSE DKNKITVAGT GSGAEFELEI DKQQGVISQW 

       790        800        810        820        830        840 
LVRGENKILQ GPKDNFFRAP LDNDIGTSEA DCIDPNAWVT QWDTAGIANL VPHCIAIEAV 

       850        860        870        880        890        900 
TLARSVLVKV EFGHYVENKL LISSHWQYTI NNQGEVQIDV NVNLAKSLSP LPRIGLELIL 

       910        920        930        940        950        960 
PDSEKPVNWF GRGPHENYPD RILSAHIARH CCSIEEMHTP YIFPSENGLR CDVKDAIVGD 

       970        980        990       1000       1010       1020 
LTVSGDFHLA VSRYSQMNIA QAKHVNDLIN DHQLYVRLDA FHMGVGGDDS WSPSVHDEFL 

      1030 
LNKEHYHYQM ILAFN 

« Hide

References

[1]"Complete sequence of Psychromonas ingrahamii 37."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 37.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000510 Genomic DNA. Translation: ABM03783.1.
RefSeqYP_943382.1. NC_008709.1.

3D structure databases

ProteinModelPortalA1SWB8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357804.Ping_2019.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM03783; ABM03783; Ping_2019.
GeneID4624702.
KEGGpin:Ping_2019.
PATRIC23069241. VBIPsyIng103130_2222.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMADPKKPYI.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycPING357804:GJBJ-2105-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_PSYIN
AccessionPrimary (citable) accession number: A1SWB8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries