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A1SU60 (SYL_PSYIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ping_1191
OrganismPsychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]
Taxonomic identifier357804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009402

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SU60 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: B0636E5861FFDE2E

FASTA85996,995
        10         20         30         40         50         60 
MQELYNPKEI EKKFQTNWDE NQSFKATEDP SKEKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMQG KNVMQPMGWD AFGLPAENAA IKNNTAPAGW TYENIAYMKT QLKQLGFGYD 

       130        140        150        160        170        180 
WSRELATCQP EYYRWEQWFF TKLVEKDLVY KKMATVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEQKEIPQ WFIKITAYAQ ELLDDLDKLD EWPEQVRTMQ RNWIGRSEGV EMDFKVSGKE 

       250        260        270        280        290        300 
ESFSVYTTRP DTVMGVTYVA VAAQHPLALE AAKNNPTLAA FCKKCKNVKL AEAEIATMAK 

       310        320        330        340        350        360 
EGVDTGFKAI HPISGLEVPI WTANFVLMGY GSGAVMSVPA HDQRDYEFAK KYDLTIKAVI 

       370        380        390        400        410        420 
KPANTDLDIS VEAYTEKGVC FDSGEFDGLD FSAAFDAVET KLVAENKGKR QVNFRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGTP IPMLNLEDGS VVPVPENELP VILPEDVQMN GIVSPIKADP DWAKTTYNGQ 

       490        500        510        520        530        540 
PATHETDTFD TFMESSWYYA RYCSPQSDDA MLDPEKANYW LPVDQYIGGI EHAILHLLYS 

       550        560        570        580        590        600 
RFFHKLLRDF GLVDCDEPYK KLLTQGMVLA DAYYYEDAKG GKVWIAPTDV ETETDEKGKV 

       610        620        630        640        650        660 
ISAATKDGQT VIYDGMSKMS KSKNNGVDPQ VMIDKYGADS VRLFMMFAAP ADQTLEWSDS 

       670        680        690        700        710        720 
ALEGSLRFLK RLWKIAFDHL ALGAVADLDL KSHTEAQKTL RRELHKTIAK VTDDIGRRQT 

       730        740        750        760        770        780 
FNTAIASVME LMNKLTKTAT KTEQDRAILQ EALLAITKLL APITPHICAE LFEVLGQTEE 

       790        800        810        820        830        840 
ILNAPWPTVD ESALVEDSKL IVVQVNGKLR AKLTVAADAQ QETVQAIAME NENVSKFTDG 

       850 
KTIRKIIFVP GKLLNIVAN 

« Hide

References

[1]"Complete sequence of Psychromonas ingrahamii 37."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 37.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000510 Genomic DNA. Translation: ABM03025.1.
RefSeqYP_942624.1. NC_008709.1.

3D structure databases

ProteinModelPortalA1SU60.
SMRA1SU60. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING357804.Ping_1191.

Proteomic databases

PRIDEA1SU60.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM03025; ABM03025; Ping_1191.
GeneID4623588.
KEGGpin:Ping_1191.
PATRIC23067375. VBIPsyIng103130_1308.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPING357804:GJBJ-1256-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PSYIN
AccessionPrimary (citable) accession number: A1SU60
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries