Reviewed, UniProtKB/Swiss-Prot A1STJ9 (SCRB_PSYIN)

Names and origin

Protein names

Recommended name:
    Probable sucrose-6-phosphate hydrolase
      Short name=Sucrase
    EC=3.2.1.26
Alternative name(s):
    Invertase

Gene names

Ordered Locus Names:

Ping_0974

Organism

Psychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]

Taxonomic identifier

357804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Psychromonadaceae › Psychromonas

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Probable sucrose-6-phosphate hydrolase		PRO_0000341296
Regions
	Region	107 – 110	4	Substrate binding By similarity
	Region	169 – 170	2	Substrate binding By similarity
	Region	230 – 231	2	Substrate binding By similarity
Sites
	Active site	110	1	By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	285	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1STJ9-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 5F715B4BA992FECB	FASTA	545	62,259
	10 20 30 40 50 60 MSLFSIIKDC GGIHNIQRVL IPDSRIIIEI HEFELLSAEA KQQAYNLTLK QVTYQQTVIE 70 80 90 100 110 120 NKDEFGAQLL EIGQLISDQQ RQDITPYTAP VECEFRPDWH ISPPQGLLND PNGFIYHQGQ 130 140 150 160 170 180 YHLFYQWYPY TCVHKDKYWA HLTSKDLVNW QWQPVALTPS DWFDSYGVFS GHAISQDDLL 190 200 210 220 230 240 MLFYTGNVRI GEQRDRHTTQ CLATSTDGIH FTKQGPVVPE LPPGVTPHCR DPKVIRHNDR 250 260 270 280 290 300 WLMLLGVQRE DEIGRLAIYH SEDLKTWTFI ALCGDELGDF GYMWECPDFF TLNQQDFIVI 310 320 330 340 350 360 GPQGIRSPDK SHTAPHHNGI VKAQLETSGK ALLSDFQPLD YGFDFYAPQS LETPDGRRIM 370 380 390 400 410 420 CAWMGLPDEI DHPSADNGWV HQLTTMRELS YDNGALIQKP IKELATLRHT PIVLSKDETS 430 440 450 460 470 480 FDLHSKAFEL QVSMQWGSVL RLHQSESGYC EIRLDSASRR LYIDRSNTLI REGDTVREMS 490 500 510 520 530 540 LAESDSVQLH IFSDTSSLEV FINEGEAVMS ARVFTDKNST QLSFDGDVQI QACWLLNKAS APFIS

References

[1]

"Complete sequence of Psychromonas ingrahamii 37." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000510 Genomic DNA. Translation: ABM02814.1.
RefSeq	YP_942413.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1STJ9.
Genome annotation databases
GeneID	4626528.
GenomeReviews	Gene locus Ping_0974 in contig CP000510_GR.
KEGG	pin:Ping_0974.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CELFESH.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_PSYIN

Accession

Primary (citable) accession number: A1STJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)