A1SSI8 (RLMF_PSYIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Ping_0599

Organism

Psychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]

Taxonomic identifier

357804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Psychromonadaceae › Psychromonas

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349943

Sequences

	Sequence		Length	Mass (Da)
	A1SSI8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 16FE810B6AD4BCFC	FASTA	310	34,853
	10 20 30 40 50 60 MTKDIQVKKL LHPRNLHRGH YDLKQLCEQS PLLSTFLRTN PKGEQTLDFA EPQAVLLLNQ 70 80 90 100 110 120 VLLKQFYHVD FWQIPKGYLC PPIPGRVDYI HYLADLLGDT FHGKIPEGKQ VKVLDIGTGA 130 140 150 160 170 180 NCIYPILGSQ SYGWSFVGTD IDPLSVKMAG LIIKSNVSLK PFIKVQLQAN KQAIFAGIIK 190 200 210 220 230 240 PKDKFTLTMC NPPFHASMEK ALAGSARKIK NLSNDQNNLS RVLNFAGQEG ELCCAGGEIR 250 260 270 280 290 300 FLKQMIQESK DYARQVCWFT SLVSKSDNIA PLKQQLEKVG AEHVKVIKMA QGQKVSRFIA 310 WSFLPQPQNF

References

[1]

"Complete sequence of Psychromonas ingrahamii 37." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000510 Genomic DNA. Translation: ABM02453.1.
RefSeq	YP_942052.1. NC_008709.1.
3D structure databases
ProteinModelPortal	A1SSI8.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SSI8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4624245.
GenomeReviews	Gene locus Ping_0599 in contig CP000510_GR.
KEGG	pin:Ping_0599.
PATRIC	23066027. VBIPsyIng103130_0661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
PhylomeDB	A1SSI8.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	PING357804:PING_0599-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSYIN

Accession

Primary (citable) accession number: A1SSI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program