Reviewed, UniProtKB/Swiss-Prot A1SSC6 (MAO1_PSYIN)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	Ping_0537

Organism

Psychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]

Taxonomic identifier

357804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Psychromonadaceae › Psychromonas

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	NAD-dependent malic enzyme HAMAP MF_01619		PRO_1000069536
Sites
	Active site	100	1	Proton donor By similarity
	Active site	171	1	Proton acceptor By similarity
	Metal binding	242	1	Divalent metal cation By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	266	1	Divalent metal cation By similarity
	Binding site	153	1	NAD By similarity
	Binding site	266	1	NAD By similarity
	Binding site	413	1	NAD By similarity
	Site	266	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SSC6-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: CAE729D46EA5CA06	FASTA	560	62,299
	10 20 30 40 50 60 MTFQRPLYIP FAGPALLESP LLNKGSAFSQ EERNNFNLTG LLPHNIETIE SQSTRAYQQL 70 80 90 100 110 120 SSFKSDLDKH IYLRNIQDTN ETLFHHLIEN HLEEVMPLIY TPTVGLACEQ FSKIYRRKRG 130 140 150 160 170 180 LFVSYPERHK IDDMLQNATK QNVKVIVVTD GERILGLGDQ GIGGMGIPIG KLALYTACGG 190 200 210 220 230 240 ISPAYCLPIV LDVGTNNSQL LADPMYMGWR NPRITGEEYN EFVDLFIQAV KRRWPEVLLQ 250 260 270 280 290 300 FEDFAQTNAT PLLNKYRDQI CCFNDDIQGT AAVSVGTLIA ACQNKGEKLS DQRIAFLGSG 310 320 330 340 350 360 SAGCGIAQHI VRQMQREGLT QEQARQRVFM VDRYGLLTDQ MTNLQAFQQP LIQYTKDLSH 370 380 390 400 410 420 WDISSHIGLE QVIKQGNISV LFGVSGQPGL FTQEVVESLC ANVDHPIVLP LSNPTSRVEA 430 440 450 460 470 480 TPKDITTWSH GQAIVATGSP FPPTLYENEY IEVSQCNNSY IFPGIGLGVL AARATAISDN 490 500 510 520 530 540 MLMAASQALA DASMQYEKVK GALLPPLGEI RPISKSIAYA VAKQAIADGL ALPVSEETMQ 550 560 RRLVDNFWAP KYRTYRRTSF

References

[1]

"Complete sequence of Psychromonas ingrahamii 37." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000510 Genomic DNA. Translation: ABM02391.1.
RefSeq	YP_941990.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4623724.
GenomeReviews	Gene locus Ping_0537 in contig CP000510_GR.
KEGG	pin:Ping_0537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1SSC6. IQDNNET.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_PSYIN

Accession

Primary (citable) accession number: A1SSC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)