Reviewed, UniProtKB/Swiss-Prot A1SS83 (SYQ_PSYIN)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	Ping_0492

Organism

Psychromonas ingrahamii (strain 37) [Complete proteome] [HAMAP]

Taxonomic identifier

357804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Psychromonadaceae › Psychromonas

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000095504
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	269 – 273	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	272	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SS83-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 06B3ED18B1F637FC	FASTA	553	63,197
	10 20 30 40 50 60 MTQVDTAPSN FIRNIIDSDL QSGKHQVIHT RFPPEPNGYL HIGHAKSICL NFGLAEDYPN 70 80 90 100 110 120 ALCNLRFDDT NPVKEDADYV AAIEKDVRWL GFKWAGDIHY SSDYFEQLYD FAIELIKKDK 130 140 150 160 170 180 AYVCALDAQQ TREYRGSLMT VGKDSPFRNR PISESLDLFA RMRAGEFAEG SMMLRAKIDM 190 200 210 220 230 240 ASPNMKMRDP VLYRIRFAHH HQTGDKWCIY PMYDFTHCIS DALEGITHSL CTLEFEDHRP 250 260 270 280 290 300 LYDWVLANIS IACKPRQIEF SRLNLQYTLT SKRKLHTLIE EKIISAWDDP RMPTISGMRR 310 320 330 340 350 360 RGYPAASIRE FCKRIGVTKQ ENTVELASLE ACVRENLEAN APRAMAVIDP VKVIISNYDE 370 380 390 400 410 420 NKTEKLIAAI HPKDESMGTR TVTFSREIFI DRADFREEAN KKYKRLVLGK EVRLRNAYVI 430 440 450 460 470 480 KADQVIKNDA GEITEIHCSY DPETLGNNPT DGRKVKGVIH WVSAVENQAA EFRIYEPLFL 490 500 510 520 530 540 VENPAAAENI DEVLNPNALV IKHGFVESGL SDALAEKAYQ FEREGYFCAD NKDSSPERLV 550 FNRTVALRDS WGG

References

[1]

"Complete sequence of Psychromonas ingrahamii 37." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Ivanova N., Staley J., Richardson P. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000510 Genomic DNA. Translation: ABM02348.1.
RefSeq	YP_941947.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1SS83.
Genome annotation databases
GeneID	4626872.
GenomeReviews	Gene locus Ping_0492 in contig CP000510_GR.
KEGG	pin:Ping_0492.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RMPTIAG.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_PSYIN

Accession

Primary (citable) accession number: A1SS83

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)