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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Psychromonas ingrahamii (strain 37)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
Binding sitei99 – 991S-adenosyl-L-methionineUniRule annotation
Binding sitei126 – 1261S-adenosyl-L-methionineUniRule annotation
Active sitei149 – 1491By similarity
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPING357804:GJBJ-400-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Ping_0377
OrganismiPsychromonas ingrahamii (strain 37)
Taxonomic identifieri357804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
ProteomesiUP000000639 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243tRNA (guanine-N(7)-)-methyltransferasePRO_0000288206Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi357804.Ping_0377.

Structurei

3D structure databases

ProteinModelPortaliA1SRX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 2244Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1SRX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQEHSASE ENADSVEKHK YMRKIRSFVK REGRMTNRQQ TAIDTLWDTM
60 70 80 90 100
GIDFEEKLID FNALFGRQAP IVLEIGFGMG KSLIEMAKNA PEKNFIGIEV
110 120 130 140 150
HGPGVGACLA DAGEAGVTNL RVINHDAVEV LDKMIPDNSL AVFQLYFPDP
160 170 180 190 200
WHKARHHKRR IVQPHFIENM RHKLAIGGVI HMATDWENYA EHMLAVLQAS
210 220 230 240
PDFKNTSESD YAPRPEWRPL TKFENRGNNL GHGVWDLLFE RIS
Length:243
Mass (Da):27,732
Last modified:February 5, 2007 - v1
Checksum:i54D4D71429C1CA02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM02238.1.
RefSeqiYP_941837.1. NC_008709.1.

Genome annotation databases

EnsemblBacteriaiABM02238; ABM02238; Ping_0377.
KEGGipin:Ping_0377.
PATRICi23065500. VBIPsyIng103130_0410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM02238.1.
RefSeqiYP_941837.1. NC_008709.1.

3D structure databases

ProteinModelPortaliA1SRX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357804.Ping_0377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM02238; ABM02238; Ping_0377.
KEGGipin:Ping_0377.
PATRICi23065500. VBIPsyIng103130_0410.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciPING357804:GJBJ-400-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 37.

Entry informationi

Entry nameiTRMB_PSYIN
AccessioniPrimary (citable) accession number: A1SRX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 28, 2007
Last sequence update: February 5, 2007
Last modified: March 31, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.