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Protein

Argininosuccinate synthase

Gene

argG

Organism
Psychromonas ingrahamii (strain 37)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei90 – 901CitrullineUniRule annotation
Binding sitei120 – 1201ATP; via amide nitrogenUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei127 – 1271AspartateUniRule annotation
Binding sitei130 – 1301CitrullineUniRule annotation
Binding sitei179 – 1791CitrullineUniRule annotation
Binding sitei188 – 1881CitrullineUniRule annotation
Binding sitei264 – 2641CitrullineUniRule annotation
Binding sitei276 – 2761CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPING357804:GJBJ-243-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Ping_0231
OrganismiPsychromonas ingrahamii (strain 37)
Taxonomic identifieri357804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesPsychromonadaceaePsychromonas
ProteomesiUP000000639 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 402402Argininosuccinate synthasePRO_0000321317Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi357804.Ping_0231.

Structurei

3D structure databases

ProteinModelPortaliA1SRI3.
SMRiA1SRI3. Positions 6-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1SRI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVKKVVLA YSGGLDTSAI IPWLKETYDD CEIIAFCADV GQGAEELVGL
60 70 80 90 100
EEKALASGAS ECHIVDLKEE FVKDYIYPTM ASGAVYEGTY LLGTAMARPI
110 120 130 140 150
IAKAQVEVAL KVGADALCHG CTGKGNDQIR FESCFAALAP ELQVIAPWRE
160 170 180 190 200
WEMESREDLL DYLAERNIET TASATKIYSR DANAWHISHE GGELENPWNA
210 220 230 240 250
PSKGVWTLTV DPEDAPDKPE YLTIKVENAR VVAVDGVELS PYDALMLLNE
260 270 280 290 300
KAAAHGVGRV DITENRTVGM KSRGCYETPG GTVMVAALRG IDELVHDKPA
310 320 330 340 350
RKWRDQVGQE FTHLVYDGRW FTPLCGSLLA ASEALAKDVN GEVVVKMYKG
360 370 380 390 400
QVTAEQKRSP NSLYSEEFAT FGDDNVYNQK HAEGFIRLYS LSSRIRTLNS

KK
Length:402
Mass (Da):44,241
Last modified:February 6, 2007 - v1
Checksum:i2F8C6406E27464AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM02098.1.
RefSeqiWP_011768657.1. NC_008709.1.

Genome annotation databases

EnsemblBacteriaiABM02098; ABM02098; Ping_0231.
KEGGipin:Ping_0231.
PATRICi23065158. VBIPsyIng103130_0250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000510 Genomic DNA. Translation: ABM02098.1.
RefSeqiWP_011768657.1. NC_008709.1.

3D structure databases

ProteinModelPortaliA1SRI3.
SMRiA1SRI3. Positions 6-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357804.Ping_0231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM02098; ABM02098; Ping_0231.
KEGGipin:Ping_0231.
PATRICi23065158. VBIPsyIng103130_0250.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciPING357804:GJBJ-243-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 37.

Entry informationi

Entry nameiASSY_PSYIN
AccessioniPrimary (citable) accession number: A1SRI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 6, 2007
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.