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A1SPW6 (PYRE_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:Noca_4354
OrganismNocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]
Taxonomic identifier196162 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_1000138809

Regions

Region115 – 12395-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site2415-phosphoribose 1-diphosphate By similarity
Binding site8915-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site9015-phosphoribose 1-diphosphate By similarity
Binding site9315-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1191Orotate By similarity
Binding site1471Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SPW6 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 8F8ECED47769EE3C

FASTA17918,851
        10         20         30         40         50         60 
MTLDATLAAD IDAVCRLTGQ FTLRSGQQAT EYFDKYLFEA DPQLLLRVAR EMVGLLPDGT 

        70         80         90        100        110        120 
DLLGGLELGG VPIATMVSSL TGRPALYVRK KAKEYGTCKL AEGPDVAGRR VTLIEDVITT 

       130        140        150        160        170 
GGAVRDATRA LRAAGAEVDV VVCAIDRSPA GEHPLADVGL EVRPVLTKAE LDAAREAGA

« Hide

References

[1]"Complete sequence of chromosome 1 of Nocardioides sp. JS614."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BAA-499 / JS614.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000509 Genomic DNA. Translation: ABL83851.1.
RefSeqYP_925538.1. NC_008699.1.

3D structure databases

ProteinModelPortalA1SPW6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1SPW6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4596872.
GenomeReviewsGene locus Noca_4354 in contig CP000509_GR.
KEGGnca:Noca_4354.
PATRIC22750853. VBINocSp122728_4653.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0461.
HOGENOMHBG404341.
OMAEMGGIPI.
PhylomeDBA1SPW6.
ProtClustDBCLSK2486794.

Enzyme and pathway databases

BioCycNSP35761:NOCA_4354-MONOMER.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_NOCSJ
AccessionPrimary (citable) accession number: A1SPW6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 6, 2007
Last modified: December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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