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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Nocardioides sp. (strain ATCC BAA-499 / JS614)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 4711D-inositol 3-phosphateUniRule annotation
Binding sitei61 – 611UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei116 – 11611D-inositol 3-phosphateUniRule annotation
Binding sitei149 – 14911D-inositol 3-phosphateUniRule annotation
Binding sitei173 – 17311D-inositol 3-phosphateUniRule annotation
Binding sitei193 – 19311D-inositol 3-phosphateUniRule annotation
Binding sitei267 – 2671UDP-GlcNAcUniRule annotation
Binding sitei272 – 2721UDP-GlcNAcUniRule annotation
Binding sitei339 – 3391UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi351 – 3511Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei361 – 3611UDP-GlcNAcUniRule annotation
Binding sitei369 – 3691UDP-GlcNAcUniRule annotation
Metal bindingi375 – 3751MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciNSP196162:GH4V-4100-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:Noca_4050
OrganismiNocardioides sp. (strain ATCC BAA-499 / JS614)
Taxonomic identifieri196162 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesNocardioidaceaeNocardioides
Proteomesi
  • UP000000640 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458D-inositol 3-phosphate glycosyltransferasePRO_0000400149Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196162.Noca_4050.

Structurei

3D structure databases

ProteinModelPortaliA1SP12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 542UDP-GlcNAc bindingUniRule annotation
Regioni58 – 6361D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A1SP12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRADRPGHRS RGINPGPGMF TLVGPDERDD PEVAVDPIRR VAMISLHTSP
60 70 80 90 100
LDQPGTGDAG GMNVYVIELS KRLAAQGIAV DIFTRATTSA VEPLVEAYDG
110 120 130 140 150
VQVRHIHAGP FEGLTKAELP GQLCVFAREV LRAEAAQPVG HYDVVHSHYW
160 170 180 190 200
LSGQVGALAR DRWGVPLVHS MHTMAKVKND ALAEGDTPEP AARIIGEEQV
210 220 230 240 250
VEAADMLVAN TDIEAKQLVN MYDADPSRVE VVHPGVDLGV FRPQDRSTAR
260 270 280 290 300
ARLGLPEDAA VLLFAGRIQP LKAPDVLLRA VAELLAQTPE LRSRLVVPIV
310 320 330 340 350
GGPSGSGLEH PESLAQLASE LGLDGAGGTG PVVRFVPPVS QEELARWCAA
360 370 380 390 400
ATLVAVPSYN ESFGLVAAEA QATGTPVVAA AVGGLTTVVR DGRSGLLVDT
410 420 430 440 450
HDPRDWADAL RRVVENDAFR DRLAAGALEQ ARLFSWEHTA RQTLDVYRRA

RAEIREAV
Length:458
Mass (Da):49,007
Last modified:February 6, 2007 - v1
Checksum:iD3F5F47DCEE07E92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL83547.1.

Genome annotation databases

EnsemblBacteriaiABL83547; ABL83547; Noca_4050.
KEGGinca:Noca_4050.
PATRICi22750233. VBINocSp122728_4347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL83547.1.

3D structure databases

ProteinModelPortaliA1SP12.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196162.Noca_4050.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL83547; ABL83547; Noca_4050.
KEGGinca:Noca_4050.
PATRICi22750233. VBINocSp122728_4347.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciNSP196162:GH4V-4100-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_NOCSJ
AccessioniPrimary (citable) accession number: A1SP12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.