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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Nocardioides sp. (strain ATCC BAA-499 / JS614)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciNSP196162:GH4V-3892-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:Noca_3843
OrganismiNocardioides sp. (strain ATCC BAA-499 / JS614)
Taxonomic identifieri196162 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesNocardioidaceaeNocardioides
Proteomesi
  • UP000000640 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310tRNA dimethylallyltransferasePRO_1000020628Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei106 – 1061Interaction with substrate tRNAUniRule annotation
Sitei127 – 1271Interaction with substrate tRNAUniRule annotation

Protein-protein interaction databases

STRINGi196162.Noca_3843.

Structurei

3D structure databases

ProteinModelPortaliA1SNF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 226Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiARKQLTW.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A1SNF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPTPNRTPI VAVVGATASG KTGLSLDLAE RLRGEIVNTD AMQVYRGMDI
60 70 80 90 100
GTAKLPVAER RGIPHHLLDT LTVRDPATVA EFQSWARAEI AEIRGRGRTP
110 120 130 140 150
VLVGGSALYT RAILDRFEFP GTDEAVRRRL EAELDALGPA ALHERLRAVD
160 170 180 190 200
PEAATRILVE NGRRIVRALE VVEITGRPFT ASLPTMEYAD PRTVQVGVDI
210 220 230 240 250
DRPTLDARIV ARVDAMFAAG FVEEVERLLA EGLAEGRTAG RAIGYREVAS
260 270 280 290 300
YLTGELSLAE ARERTVSATR RFSRRQDSWF RKDPRVTWIR YDDPGLVDRA
310
VSVVAATLDG
Length:310
Mass (Da):33,993
Last modified:February 6, 2007 - v1
Checksum:i268B20CDD4FB7E57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL83341.1.
RefSeqiWP_011757272.1. NC_008699.1.

Genome annotation databases

EnsemblBacteriaiABL83341; ABL83341; Noca_3843.
KEGGinca:Noca_3843.
PATRICi22749801. VBINocSp122728_4132.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL83341.1.
RefSeqiWP_011757272.1. NC_008699.1.

3D structure databases

ProteinModelPortaliA1SNF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196162.Noca_3843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL83341; ABL83341; Noca_3843.
KEGGinca:Noca_3843.
PATRICi22749801. VBINocSp122728_4132.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiARKQLTW.
OrthoDBiPOG091H00NK.

Enzyme and pathway databases

BioCyciNSP196162:GH4V-3892-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_NOCSJ
AccessioniPrimary (citable) accession number: A1SNF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.