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A1SMR4 (CHEB_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chemotaxis response regulator protein-glutamate methylesterase

EC=3.1.1.61
Gene names
Name:cheB
Ordered Locus Names:Noca_3599
OrganismNocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]
Taxonomic identifier196162 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP-Rule MF_00099

Catalytic activity

Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP-Rule MF_00099

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00099.

Domain

The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain By similarity. HAMAP-Rule MF_00099

Post-translational modification

Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP-Rule MF_00099

Sequence similarities

Contains 1 cheB-type methylesterase domain.

Contains 1 response regulatory domain.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCytoplasm
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processchemotaxis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

sensory perception of chemical stimulus

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphorelay response regulator activity

Inferred from electronic annotation. Source: InterPro

protein-glutamate methylesterase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Chemotaxis response regulator protein-glutamate methylesterase HAMAP-Rule MF_00099
PRO_1000075591

Regions

Domain5 – 123119Response regulatory
Domain163 – 355193CheB-type methylesterase

Sites

Active site1751 By similarity
Active site2011 By similarity
Active site2971 By similarity

Amino acid modifications

Modified residue5614-aspartylphosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SMR4 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: A8548AC96A3B7794

FASTA36638,224
        10         20         30         40         50         60 
MPGIRVLVVD DSVVIRKLVS DLLSADPDIE VVGTAVNGRA ALQKVAQLRP DLVTMDIEMP 

        70         80         90        100        110        120 
EMDGIESVRA IRATGNKVPI IMFSTLTERG AVATLDALGA GASDYVPKPA NVGSVGRSME 

       130        140        150        160        170        180 
QVREALIPRI KSLVPRRGIT PHPAGVTVAP TATVQLHAPA TPPLGGHRLL VIGSSTGGPE 

       190        200        210        220        230        240 
ALSALLHTLP PLSVPVAVVQ HMPPLFTRQF AARLDRQLPY DVVEAEHNQP LRPGTVAIAP 

       250        260        270        280        290        300 
GDYHLEVTTD AAGLRTRLTQ APPENYCRPA VDVLFRSAVA AVGPAVLAAV LTGMGSDGCK 

       310        320        330        340        350        360 
GARLIVERGG SVLAQDQATS VVWGMPGAVA TAGLAERVLP LLELGPEILR RLARQRPSLV 


PAGALS 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nocardioides sp. JS614."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BAA-499 / JS614.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000509 Genomic DNA. Translation: ABL83099.1.
RefSeqYP_924786.1. NC_008699.1.

3D structure databases

ProteinModelPortalA1SMR4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196162.Noca_3599.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL83099; ABL83099; Noca_3599.
GeneID4599402.
KEGGnca:Noca_3599.
PATRIC22749301. VBINocSp122728_3882.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2201.
HOGENOMHOG000151424.
KOK03412.
OMAEVVDLHQ.
OrthoDBEOG6PKFC7.

Enzyme and pathway databases

BioCycNSP196162:GH4V-3648-MONOMER.

Family and domain databases

Gene3D3.40.50.180. 1 hit.
HAMAPMF_00099. CheB_methylest.
InterProIPR011006. CheY-like_superfamily.
IPR008248. Sig_transdc_resp-reg_CheB.
IPR000673. Sig_transdc_resp-reg_Me-estase.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamPF01339. CheB_methylest. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PIRSFPIRSF000876. RR_chemtxs_CheB. 1 hit.
SMARTSM00448. REC. 1 hit.
[Graphical view]
SUPFAMSSF52172. SSF52172. 1 hit.
SSF52738. SSF52738. 1 hit.
PROSITEPS50122. CHEB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHEB_NOCSJ
AccessionPrimary (citable) accession number: A1SMR4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families