A1SMR4 (CHEB_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase
EC=3.1.1.61

Gene names

Name:	cheB
Ordered Locus Names:	Noca_3599

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP-Rule MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP-Rule MF_00099
Subcellular location	Cytoplasm By similarity.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain By similarity. HAMAP-Rule MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP-Rule MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from electronic annotation. Source: HAMAP intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Chemotaxis response regulator protein-glutamate methylesterase HAMAP-Rule MF_00099		PRO_1000075591
Regions
	Domain	5 – 123	119	Response regulatory
	Domain	163 – 355	193	CheB-type methylesterase
Sites
	Active site	175	1	By similarity
	Active site	201	1	By similarity
	Active site	297	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SMR4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A8548AC96A3B7794	FASTA	366	38,224
	10 20 30 40 50 60 MPGIRVLVVD DSVVIRKLVS DLLSADPDIE VVGTAVNGRA ALQKVAQLRP DLVTMDIEMP 70 80 90 100 110 120 EMDGIESVRA IRATGNKVPI IMFSTLTERG AVATLDALGA GASDYVPKPA NVGSVGRSME 130 140 150 160 170 180 QVREALIPRI KSLVPRRGIT PHPAGVTVAP TATVQLHAPA TPPLGGHRLL VIGSSTGGPE 190 200 210 220 230 240 ALSALLHTLP PLSVPVAVVQ HMPPLFTRQF AARLDRQLPY DVVEAEHNQP LRPGTVAIAP 250 260 270 280 290 300 GDYHLEVTTD AAGLRTRLTQ APPENYCRPA VDVLFRSAVA AVGPAVLAAV LTGMGSDGCK 310 320 330 340 350 360 GARLIVERGG SVLAQDQATS VVWGMPGAVA TAGLAERVLP LLELGPEILR RLARQRPSLV PAGALS

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL83099.1.
RefSeq	YP_924786.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SMR4.
ModBase	Search...
Protein-protein interaction databases
STRING	196162.Noca_3599.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABL83099; ABL83099; Noca_3599.
GeneID	4599402.
KEGG	nca:Noca_3599.
PATRIC	22749301. VBINocSp122728_3882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HOG000151424.
KO	K03412.
Enzyme and pathway databases
BioCyc	NSP196162:GH4V-3654-MONOMER.
Family and domain databases
Gene3D	3.40.50.180. 1 hit.
HAMAP	MF_00099. CheB_methylest.
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB_NOCSJ

Accession

Primary (citable) accession number: A1SMR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program