Reviewed, UniProtKB/Swiss-Prot A1SMP7 (FOLD2_NOCSJ)

Names and origin

Protein names

Recommended name:
    Bifunctional protein folD 2
Including the following 2 domains:
    1- Recommended name:
            Methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.5
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:	folD2
Ordered Locus Names:	Noca_3582

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity. HAMAP MF_01576
Catalytic activity	5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP MF_01576 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP MF_01576
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01576
Subunit structure	Homodimer By similarity. HAMAP MF_01576
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis Methionine biosynthesis One-carbon metabolism Purine biosynthesis
Ligand	NADP
Molecular function	Hydrolase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Bifunctional protein folD 2 HAMAP MF_01576		PRO_0000305854

Sequences

	Sequence		Length	Mass (Da)
	A1SMP7-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: F66FF1AEAD723F64	FASTA	283	29,369
	10 20 30 40 50 60 MTAQKLDGTA TAAAIKGELT TRVAALHERG IRPGLGTILV GDDPGSRWYV NGKHKDCAEV 70 80 90 100 110 120 GIESIRIDLP ATATQDEIED AVRVLNDDPA CTGYIVQLPL PKGRDENRVL GLIDPAKDAD 130 140 150 160 170 180 GLHPTNLGWL VLGKAAPLPC TPNGIVELLR RHGVEIAGAE VTVVGRGVTV GRPLGLLLTR 190 200 210 220 230 240 RSENATVTLC HTGTRDLAAH VRGADIVVAA AGVPGIVTGA MVKPGAAVLD VGVSRVDGKL 250 260 270 280 AGDVAPEVWD VARWVSPNPG GVGPMTRAML LSNIVAMAEQ TDS

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL83082.1.
RefSeq	YP_924769.1.
3D structure databases
SMR	A1SMP7. Positions 6-280.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SMP7.
Genome annotation databases
GeneID	4599461.
GenomeReviews	Gene locus Noca_3582 in contig CP000509_GR.
KEGG	nca:Noca_3582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0190.
HOGENOM	HBG328751.
OMA	DVVYETA.
Family and domain databases
HAMAP	MF_01576. THF_DHG_CYH. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
PROSITE	PS00766. THF_DHG_CYH_1. False negative. PS00767. THF_DHG_CYH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOLD2_NOCSJ

Accession

Primary (citable) accession number: A1SMP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	February 6, 2007
Last modified:	January 19, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)