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Protein

Malate dehydrogenase

Gene

mdh

Organism
Nocardioides sp. (strain ATCC BAA-499 / JS614)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateUniRule annotation1
Binding sitei98SubstrateUniRule annotation1
Binding sitei105NADUniRule annotation1
Binding sitei112NADUniRule annotation1
Binding sitei131SubstrateUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Active sitei187Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADUniRule annotation7
Nucleotide bindingi129 – 131NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Noca_3578
OrganismiNocardioides sp. (strain ATCC BAA-499 / JS614)
Taxonomic identifieri196162 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesNocardioidaceaeNocardioides
Proteomesi
  • UP000000640 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002943971 – 328Malate dehydrogenaseAdd BLAST328

Proteomic databases

PRIDEiA1SMP3.

Interactioni

Protein-protein interaction databases

STRINGi196162.Noca_3578.

Structurei

3D structure databases

ProteinModelPortaliA1SMP3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiPOG091H03R4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1SMP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTPLKVAV TGAAGQIGYS LLFRLASGSL LGDRPIELRL LEITPALKAL
60 70 80 90 100
EGVVMELDDC AFPNLAGVQI GDDAEQIFDG VNLALLVGAR PRGPGMERGD
110 120 130 140 150
LLSANGAIFT AQGKALNKVA ADDVRIGVTG NPANTNALIA MTNAPDIPQA
160 170 180 190 200
RFSALTRLDH NRAISQLAAK TGAAVTDIKK MTIWGNHSAT QYPDVFHAEI
210 220 230 240 250
GGRNAAEVVG DQDWIESTFI PTVAKRGAAI IEARGSSSAA SAASATIDAA
260 270 280 290 300
RDWLFGSADA DWVSMAVVSD GSYGVPEGLI SSFPVTTKDG DWEIVQGLEI
310 320
DDFSRAKIDA STAELADERE AVKELGLI
Length:328
Mass (Da):34,261
Last modified:February 6, 2007 - v1
Checksum:i1AA000686E98A0B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL83078.1.
RefSeqiWP_011757009.1. NC_008699.1.

Genome annotation databases

EnsemblBacteriaiABL83078; ABL83078; Noca_3578.
KEGGinca:Noca_3578.
PATRICi22749259. VBINocSp122728_3861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL83078.1.
RefSeqiWP_011757009.1. NC_008699.1.

3D structure databases

ProteinModelPortaliA1SMP3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196162.Noca_3578.

Proteomic databases

PRIDEiA1SMP3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL83078; ABL83078; Noca_3578.
KEGGinca:Noca_3578.
PATRICi22749259. VBINocSp122728_3861.

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000220953.
KOiK00024.
OMAiAFSQECI.
OrthoDBiPOG091H03R4.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_NOCSJ
AccessioniPrimary (citable) accession number: A1SMP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.