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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Nocardioides sp. (strain BAA-499 / JS614)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei101 – 1011NucleophileUniRule annotation
Binding sitei125 – 1251ATP; via amide nitrogenUniRule annotation
Active sitei198 – 1981Cysteine persulfide intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 138ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciNSP196162:GH4V-3492-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.13UniRule annotation)
Gene namesi
Name:mnmAUniRule annotation
Ordered Locus Names:Noca_3445
OrganismiNocardioides sp. (strain BAA-499 / JS614)
Taxonomic identifieri196162 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesNocardioidaceaeNocardioides
Proteomesi
  • UP000000640 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366tRNA-specific 2-thiouridylase MnmAPRO_0000349722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi101 ↔ 198AlternateUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei126 – 1261Interaction with tRNAUniRule annotation
Sitei341 – 3411Interaction with tRNAUniRule annotation

Protein-protein interaction databases

STRINGi196162.Noca_3445.

Structurei

3D structure databases

ProteinModelPortaliA1SMB0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1503Interaction with tRNAUniRule annotation

Sequence similaritiesi

Belongs to the MnmA/TRMU family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218045.
KOiK00566.
OMAiNPQSFRT.
OrthoDBiEOG6RZB5H.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

A1SMB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLAAMSGG VDSAVAAARA AEAGHDVTGI HLALSRNPQS YRSGARGCCT
60 70 80 90 100
IEDSNDARRA ADVIGIPFYV WDLSDRFHED VVEDFMAEYA AGRTPNPCLR
110 120 130 140 150
CNEKIKFAAV LDRALGLGFD AVATGHYARL VAGADGLVEM HRAVDHGKDQ
160 170 180 190 200
SYVLGVLDQG QLRHSLFPLG GSTKDEVRRE AAERGLLVAD KPDSHDICFI
210 220 230 240 250
ADGDNAGWLR EKLGDRAPHH EGTIVDDDTG EVLGRHEGSY GFTIGQRKGL
260 270 280 290 300
RIGRPAPDGR PRFVLDIEPV SGTVTVGPHE RLAVHRLRGI RPRWCGTVPD
310 320 330 340 350
RLSGTVQLRA HGEEHRAVLV RDGDEVDIEL LEPAYGIAPG QAAVLYDGSR
360
VVGSATISAT DRVAAR
Length:366
Mass (Da):39,397
Last modified:February 6, 2007 - v1
Checksum:iAD85436D89F8A5AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL82945.1.
RefSeqiWP_011756878.1. NC_008699.1.

Genome annotation databases

EnsemblBacteriaiABL82945; ABL82945; Noca_3445.
KEGGinca:Noca_3445.
PATRICi22748999. VBINocSp122728_3733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL82945.1.
RefSeqiWP_011756878.1. NC_008699.1.

3D structure databases

ProteinModelPortaliA1SMB0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196162.Noca_3445.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL82945; ABL82945; Noca_3445.
KEGGinca:Noca_3445.
PATRICi22748999. VBINocSp122728_3733.

Phylogenomic databases

eggNOGiENOG4105CCJ. Bacteria.
COG0482. LUCA.
HOGENOMiHOG000218045.
KOiK00566.
OMAiNPQSFRT.
OrthoDBiEOG6RZB5H.

Enzyme and pathway databases

BioCyciNSP196162:GH4V-3492-MONOMER.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nocardioides sp. JS614."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F.
    , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BAA-499 / JS614.

Entry informationi

Entry nameiMNMA_NOCSJ
AccessioniPrimary (citable) accession number: A1SMB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 6, 2007
Last modified: March 16, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.