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A1SM80

- BIOB_NOCSJ

UniProt

A1SM80 - BIOB_NOCSJ

Protein

Biotin synthase

Gene

bioB

Organism
Nocardioides sp. (strain BAA-499 / JS614)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi273 – 2731Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciNSP196162:GH4V-3462-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Noca_3415
    OrganismiNocardioides sp. (strain BAA-499 / JS614)
    Taxonomic identifieri196162 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides
    ProteomesiUP000000640: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 374374Biotin synthasePRO_0000381509Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196162.Noca_3415.

    Structurei

    3D structure databases

    ProteinModelPortaliA1SM80.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1SM80-1 [UniParc]FASTAAdd to Basket

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    MQTSFDHLAD RILAGGDATP ADALAVLRAD EKDLLHVVAA AGRLRRARFG    50
    NTVKVNYLVN LKSGLCPEDC HYCSQALGSR APILKYNWLS SEEVLEQAGA 100
    GLRGGATRVC LVSSGRGPSD RDVDRVAAMA QELKGEQPGV EICACLGLLK 150
    DGQAERLRAA GVDAYNHNIN TAESHHDTIV STHSYSDRVD TIEKAAAAGL 200
    SPCSGLIAGL GETDEQLVEA LFALKALGAD SIPVNFLMPF DGTPSERTFE 250
    LTPIRCVQIL AMTRFVCPDT EIRIAGGREM HLRSLQALAL HVANSIFLGD 300
    YLTSEGQDAR ADLEMLRDNG FAILGAEAKP AGTATAAHRA QTAHDIAGGT 350
    SVAGSAPDPA IRRRGAGTDV PANA 374
    Length:374
    Mass (Da):39,431
    Last modified:February 6, 2007 - v1
    Checksum:i657D95068D6F6A8F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000509 Genomic DNA. Translation: ABL82915.1.
    RefSeqiWP_011756849.1. NC_008699.1.
    YP_924602.1. NC_008699.1.

    Genome annotation databases

    EnsemblBacteriaiABL82915; ABL82915; Noca_3415.
    GeneIDi4598213.
    KEGGinca:Noca_3415.
    PATRICi22748933. VBINocSp122728_3700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000509 Genomic DNA. Translation: ABL82915.1 .
    RefSeqi WP_011756849.1. NC_008699.1.
    YP_924602.1. NC_008699.1.

    3D structure databases

    ProteinModelPortali A1SM80.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196162.Noca_3415.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL82915 ; ABL82915 ; Noca_3415 .
    GeneIDi 4598213.
    KEGGi nca:Noca_3415.
    PATRICi 22748933. VBINocSp122728_3700.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci NSP196162:GH4V-3462-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Nocardioides sp. JS614."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F.
      , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
      Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BAA-499 / JS614.

    Entry informationi

    Entry nameiBIOB_NOCSJ
    AccessioniPrimary (citable) accession number: A1SM80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3