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A1SM03

- SYE_NOCSJ

UniProt

A1SM03 - SYE_NOCSJ

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Nocardioides sp. (strain BAA-499 / JS614)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi111 – 1111ZincUniRule annotation
    Metal bindingi113 – 1131ZincUniRule annotation
    Metal bindingi136 – 1361ZincUniRule annotation
    Metal bindingi138 – 1381ZincUniRule annotation
    Binding sitei260 – 2601ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciNSP196162:GH4V-3383-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Noca_3336
    OrganismiNocardioides sp. (strain BAA-499 / JS614)
    Taxonomic identifieri196162 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides
    ProteomesiUP000000640: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Glutamate--tRNA ligasePRO_0000330986Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196162.Noca_3336.

    Structurei

    3D structure databases

    ProteinModelPortaliA1SM03.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi14 – 2411"HIGH" regionAdd
    BLAST
    Motifi257 – 2615"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiKHYDGDF.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1SM03-1 [UniParc]FASTAAdd to Basket

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    MNNQPKTVRV RMAPSPTGSP HVGLVRTALF NWAFARHHHG TFVFRIEDTD    50
    RERSTQESYD AIIDLMRWLG LDWDEGPEVG GPHAPYFQSE RGDIYRDALA 100
    RLAESSYTYD CFCTNEEVDA RRKASGSKVM GYDGFCRDLS TEQRAAFEAE 150
    GRRPVVRFRM PDGSITWHDL VRGDVTFETR FVPDYALCRA NGDPLYTLVN 200
    PVDDAYMEIT HVLRGEDLLS STPRQIALYA ALEELGIAKA TPEFGHLPYV 250
    MGEGNKKLSK RDPEAHALAY RDQGFLPEGL LNYLALLGWA IAADRDVFSM 300
    EEMVAAFDIA DVNPNPARFD MKKAEAINAA HMRLLSLDVM TERVLPFLKG 350
    AGVLSDPVNP ADTQLLELAM PLVAERINKL TESVDMLGFL FVDEESFTRD 400
    EADAEKLLND DGRAVVRRAL DALERVGEWS TGAIQDALQA ELVEAMGVKP 450
    RNAFGPVRVA VTGRRVSPPL FESMELLGRD RSLARLRSAL G 491
    Length:491
    Mass (Da):54,814
    Last modified:February 6, 2007 - v1
    Checksum:iD6D5325734FD7A4C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000509 Genomic DNA. Translation: ABL82838.1.
    RefSeqiWP_011756772.1. NC_008699.1.
    YP_924525.1. NC_008699.1.

    Genome annotation databases

    EnsemblBacteriaiABL82838; ABL82838; Noca_3336.
    GeneIDi4600230.
    KEGGinca:Noca_3336.
    PATRICi22748761. VBINocSp122728_3612.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000509 Genomic DNA. Translation: ABL82838.1 .
    RefSeqi WP_011756772.1. NC_008699.1.
    YP_924525.1. NC_008699.1.

    3D structure databases

    ProteinModelPortali A1SM03.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196162.Noca_3336.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL82838 ; ABL82838 ; Noca_3336 .
    GeneIDi 4600230.
    KEGGi nca:Noca_3336.
    PATRICi 22748761. VBINocSp122728_3612.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi KHYDGDF.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci NSP196162:GH4V-3383-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Nocardioides sp. JS614."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F.
      , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
      Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BAA-499 / JS614.

    Entry informationi

    Entry nameiSYE_NOCSJ
    AccessioniPrimary (citable) accession number: A1SM03
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3