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A1SM03

- SYE_NOCSJ

UniProt

A1SM03 - SYE_NOCSJ

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Protein
Glutamate--tRNA ligase
Gene
gltX, Noca_3336
Organism
Nocardioides sp. (strain BAA-499 / JS614)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111Zinc By similarity
Metal bindingi113 – 1131Zinc By similarity
Metal bindingi136 – 1361Zinc By similarity
Metal bindingi138 – 1381Zinc By similarity
Binding sitei260 – 2601ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNSP196162:GH4V-3383-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:Noca_3336
OrganismiNocardioides sp. (strain BAA-499 / JS614)
Taxonomic identifieri196162 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides
ProteomesiUP000000640: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Glutamate--tRNA ligaseUniRule annotation
PRO_0000330986Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196162.Noca_3336.

Structurei

3D structure databases

ProteinModelPortaliA1SM03.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi14 – 2411"HIGH" regionUniRule annotation
Add
BLAST
Motifi257 – 2615"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiKHYDGDF.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A1SM03-1 [UniParc]FASTAAdd to Basket

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MNNQPKTVRV RMAPSPTGSP HVGLVRTALF NWAFARHHHG TFVFRIEDTD    50
RERSTQESYD AIIDLMRWLG LDWDEGPEVG GPHAPYFQSE RGDIYRDALA 100
RLAESSYTYD CFCTNEEVDA RRKASGSKVM GYDGFCRDLS TEQRAAFEAE 150
GRRPVVRFRM PDGSITWHDL VRGDVTFETR FVPDYALCRA NGDPLYTLVN 200
PVDDAYMEIT HVLRGEDLLS STPRQIALYA ALEELGIAKA TPEFGHLPYV 250
MGEGNKKLSK RDPEAHALAY RDQGFLPEGL LNYLALLGWA IAADRDVFSM 300
EEMVAAFDIA DVNPNPARFD MKKAEAINAA HMRLLSLDVM TERVLPFLKG 350
AGVLSDPVNP ADTQLLELAM PLVAERINKL TESVDMLGFL FVDEESFTRD 400
EADAEKLLND DGRAVVRRAL DALERVGEWS TGAIQDALQA ELVEAMGVKP 450
RNAFGPVRVA VTGRRVSPPL FESMELLGRD RSLARLRSAL G 491
Length:491
Mass (Da):54,814
Last modified:February 6, 2007 - v1
Checksum:iD6D5325734FD7A4C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000509 Genomic DNA. Translation: ABL82838.1.
RefSeqiWP_011756772.1. NC_008699.1.
YP_924525.1. NC_008699.1.

Genome annotation databases

EnsemblBacteriaiABL82838; ABL82838; Noca_3336.
GeneIDi4600230.
KEGGinca:Noca_3336.
PATRICi22748761. VBINocSp122728_3612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000509 Genomic DNA. Translation: ABL82838.1 .
RefSeqi WP_011756772.1. NC_008699.1.
YP_924525.1. NC_008699.1.

3D structure databases

ProteinModelPortali A1SM03.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196162.Noca_3336.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL82838 ; ABL82838 ; Noca_3336 .
GeneIDi 4600230.
KEGGi nca:Noca_3336.
PATRICi 22748761. VBINocSp122728_3612.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
KOi K01885.
OMAi KHYDGDF.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci NSP196162:GH4V-3383-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nocardioides sp. JS614."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F.
    , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BAA-499 / JS614.

Entry informationi

Entry nameiSYE_NOCSJ
AccessioniPrimary (citable) accession number: A1SM03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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