A1SLL4 (SYP_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Noca_3197

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Proline--tRNA ligase HAMAP MF_01569		PRO_0000288357

Sequences

	Sequence		Length	Mass (Da)
	A1SLL4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 33E793AF680FD7D4	FASTA	589	64,583
	10 20 30 40 50 60 MIMRMSSLFV RTLREDPADA EVPSHRLLVR AGYIRRAAPG IYTWLPLGLR VLRKIENVIR 70 80 90 100 110 120 EEMDAIGAQE MLFPALLPRE PYEATNRWTE YGDGIFRLQD RKGADYLLGP THEEMFTLVV 130 140 150 160 170 180 KDLYSSYKDL PLSIYQIQTK YRDEARPRAG LLRGREFVMK DSYSFDVDDA GLDVSYQKHR 190 200 210 220 230 240 DAYVRIFDRL GFEYVIVEAM SGAMGGSKSE EFLAKASVGE DTYVRCTLCD YAANVEAVHS 250 260 270 280 290 300 PPIPPVPYDD APAAHAEQTP DTPTIETLVA HLNERFPRED RPWAASDTLK NVVFSVHHPD 310 320 330 340 350 360 GATEALAVGL PGDREVDAKR LEAHLGEGVV FEPFGEADFA ARPTLAKGYI GPGALGEKKP 370 380 390 400 410 420 AGVRYLLDPR VVEGTRWVTG ADAAGSHVID LVAGRDFSGD GLIEVAEVRD GDPCPRGDGG 430 440 450 460 470 480 TLETARGIEM GHIFQLGRKY ADALDLKVLD EQGKLVTVTM GSYGIGPSRA VAAIAEGTHD 490 500 510 520 530 540 ELGLAWPREV APADVHIVAT GKDEHVFAAA ERIAHELDKQ GVEVLYDDRP KVSPGVKFKD 550 560 570 580 AELIGVPTIV VVGKGLAAGT IEVKDRRTGQ RQDVPADHLV DRVIAIVRA

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL82699.1.
RefSeq	YP_924386.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SLL4.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SLL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4599060.
GenomeReviews	Gene locus Noca_3197 in contig CP000509_GR.
KEGG	nca:Noca_3197.
PATRIC	22748473. VBINocSp122728_3470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
PhylomeDB	A1SLL4.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_3197-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_NOCSJ

Accession

Primary (citable) accession number: A1SLL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program