A1SL88 (RSMH_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	Noca_3071

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000387011
Regions
	Region	37 – 39	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	56	1	S-adenosyl-L-methionine By similarity
	Binding site	90	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	104	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SL88 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4898A6825014950F	FASTA	319	34,283
	10 20 30 40 50 60 MSGPSHVPVL LDRVVALLAP PLEREGSVLV DATLGLGGHT EAVLTRFDLA RVVGIDRDPE 70 80 90 100 110 120 ALALAGRRLA PFGDRFTGVH AVYDELPDVL ARLGLDAVDA VLFDLGVSSM QLDVRERGFA 130 140 150 160 170 180 YAEDAPLDMR MDGSTGPTAA DVLNTYAAAD LARILREYGE ERFARKIAAA VVRERAKEPF 190 200 210 220 230 240 TRSGRLVELL YAEIPAPARR TGGHPAKRTF QALRMEVNDE LAVLRRAIPA AIDAIGVGGR 250 260 270 280 290 300 VVVESYHSLE DRLVKQAFVA ASRLDVPEDL PFVPAGHEPA LRLVTRGAEK AGPEEIALNP 310 RAASVRLRAI ERTSKGAAA

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL82573.1.
RefSeq	YP_924260.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SL88.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SL88.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4600188.
GenomeReviews	Gene locus Noca_3071 in contig CP000509_GR.
KEGG	nca:Noca_3071.
PATRIC	22748213. VBINocSp122728_3340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0275.
HOGENOM	HBG302779.
OMA	NPATRAF.
PhylomeDB	A1SL88.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_3071-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_NOCSJ

Accession

Primary (citable) accession number: A1SL88

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program