A1SKS5 (A1SKS5_NOCSJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116
Short name=dUTPase HAMAP MF_00116
EC=3.6.1.23 HAMAP MF_00116

Alternative name(s):
dUTP pyrophosphatase HAMAP MF_00116

Gene names

Name:	dut HAMAP MF_00116
Ordered Locus Names:	Noca_2908

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116 SAAS SAAS008181
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116 SAAS SAAS008181
Cofactor	Magnesium By similarity. HAMAP MF_00116 SAAS SAAS008181
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family. HAMAP MF_00116

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP MF_00116 SAAS SAAS008181
Ligand	Magnesium HAMAP MF_00116 SAAS SAAS008181 Metal-binding HAMAP MF_00116 SAAS SAAS008181
Molecular function	Hydrolase HAMAP MF_00116 SAAS SAAS008181 EMBL ABL82410.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	66 – 68	3	Substrate binding By similarity HAMAP MF_00116
	Region	83 – 85	3	Substrate binding By similarity HAMAP MF_00116
Sites
	Binding site	79	1	Substrate By similarity HAMAP MF_00116
	Binding site	93	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_00116

Sequences

	Sequence		Length	Mass (Da)
	A1SKS5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C3D86E3D76505AEF	FASTA	148	15,587
	10 20 30 40 50 60 MTSDLEIPVQ RLDPGLPLPA YAHPGDAGAD LLTTVDVTLA PGERAMVPTG IALALPEGYA 70 80 90 100 110 120 AFVHPRSGLA ARHGLSIVNT PGTIDAGYRG EIKVMLVNHD PREPIELRRG DRIAQLVVQR 130 140 VERARFTEVG ELPASVRGAG GYGSTGGF

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL82410.1.
RefSeq	YP_924097.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SKS5.
SMR	A1SKS5. Positions 7-141.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SKS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4596357.
GenomeReviews	Gene locus Noca_2908 in contig CP000509_GR.
KEGG	nca:Noca_2908.
PATRIC	22747873. VBINocSp122728_3171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	KIAQMVI.
PhylomeDB	A1SKS5.
ProtClustDB	CLSK781023.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_2908-MONOMER.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1SKS5_NOCSJ

Accession

Primary (citable) accession number: A1SKS5

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)