A1SKM6 (DXS_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Noca_2859

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 634	634	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019051

Sequences

	Sequence		Length	Mass (Da)
	A1SKM6 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 37DBED42C138D745	FASTA	634	67,660
	10 20 30 40 50 60 MGLLDSIASP ADLRGLSDAQ LEQLASEIRD LLIRTVATNT GHLGPNLGVV ELTLAIHRVF 70 80 90 100 110 120 DSPRDRVVFD TGHQAYVHKL VCGRAASFGT LRREGGVSGY PSQAESPHDI VENSHASTAL 130 140 150 160 170 180 SYADGLAKAY TIRGEDRHVI AVIGDGALTG GMAWEALNNI AIAKASKLVI VVNDNGRSYT 190 200 210 220 230 240 PTIGGLATAL TSLRTNPRYE NVLDLVKRRL NAVPGVGPAA YDALHAMKKG LKDALAPQGL 250 260 270 280 290 300 FEDLGLKYVG PVDGHDRAAM EQVLAQAKRF NGPVIVHAIT RKGYGYDPAE RHEADQFHAP 310 320 330 340 350 360 GPFDVQTGAE KPKGRIWTDV FSEEIVHLGH RRKDLVGITA AMMHPVGLDA FQKHFPERTF 370 380 390 400 410 420 DVGIAEQHAA TSAAGLAMGG MHPVVALYGT FLNRAFDQVL MDVALHKCGV TFVLDRSGVT 430 440 450 460 470 480 GDDGASHNGM WDMSILQVVP GLRLAAPRDP ARLRELLNEA VEVDDAPTVV RFPKGSPPED 490 500 510 520 530 540 IEAVDRAGGA DILVRNGTKD VLVVAVGAMG TTAVDVAQRL TAQGIGVTVV DPRWVKPVDP 550 560 570 580 590 600 AIVELAREHR LVVSIEDNGE TGGCGAVLLQ TLNAAGVHTP FRLHGIPQQF LGHAKRAAIL 610 620 630 ERIGLTPQAI ALRIVEDMTS LTEGRSPLDV EQAT

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL82361.1.
RefSeq	YP_924048.1. NC_008699.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1SKM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4598458.
GenomeReviews	Gene locus Noca_2859 in contig CP000509_GR.
KEGG	nca:Noca_2859.
PATRIC	22747777. VBINocSp122728_3123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_2859-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_NOCSJ

Accession

Primary (citable) accession number: A1SKM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program