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A1SKL2 (DNLI_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Noca_2845
OrganismNocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]
Taxonomic identifier196162 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Probable DNA ligase HAMAP-Rule MF_00407
PRO_0000365236

Sites

Active site2201N6-AMP-lysine intermediate By similarity
Binding site2181ATP By similarity
Binding site2251ATP By similarity
Binding site2401ATP By similarity
Binding site2691ATP By similarity
Binding site3021ATP By similarity
Binding site3741ATP By similarity
Binding site3801ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SKL2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: D1533EF8065E8329

FASTA50953,371
        10         20         30         40         50         60 
MNGAVLIADV VATSAAITAT RSRKAKVAAI ADLLARADPR RSGADELETV TAYVGGALRQ 

        70         80         90        100        110        120 
RRTGLGWRGL TKLPAPAPGP TLSVLEVHEA FERIAAPAGA GSQAARAAAV ADLFGRATAE 

       130        140        150        160        170        180 
EQQWLRGVVT GEVRQGALDS LVQEGLAAAA GVPLPEVRRA AMLAGSTVAV AAAAFEGVAA 

       190        200        210        220        230        240 
LGAVGLQVGR PVLPMLASSA PDLAAALARA GGDEVAVDTK LDGIRIQVHR SGPDVVVATR 

       250        260        270        280        290        300 
SLEDITARLP EVVEVARALP ADSFVLDGEA LALAEDGRPR PFQETAARTA MGAGVEVTPY 

       310        320        330        340        350        360 
FFDVLHLDGA DLLDVSAAER AAVLERLVPA EHRVPRVVTA DLGVAEEFLA GALRSGHEGV 

       370        380        390        400        410        420 
VVKSLAAPYE AGRRGASWVK VKPVHTLDLV VLAVEWGSGR RQGWLSNIHL GARDPATGGL 

       430        440        450        460        470        480 
VMLGKTFKGM TDEVLAWQTE RFLELETSRS AHVVHVRPEQ VVEIAFDGVQ RSTRYPGGVA 

       490        500 
LRFARVLRYR DDKPVAEIDT VDTVRSFLG 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nocardioides sp. JS614."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BAA-499 / JS614.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000509 Genomic DNA. Translation: ABL82347.1.
RefSeqYP_924034.1. NC_008699.1.

3D structure databases

ProteinModelPortalA1SKL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196162.Noca_2845.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL82347; ABL82347; Noca_2845.
GeneID4598444.
KEGGnca:Noca_2845.
PATRIC22747747. VBINocSp122728_3108.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK01971.
OMAGRPRPFQ.
OrthoDBEOG6Q8HXT.
ProtClustDBPRK03180.

Enzyme and pathway databases

BioCycNSP196162:GH4V-2889-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_NOCSJ
AccessionPrimary (citable) accession number: A1SKL2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families