A1SIL4 (A1SIL4_NOCSJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase 1 HAMAP MF_00435
EC=1.1.1.86 HAMAP MF_00435

Alternative name(s):
Acetohydroxy-acid isomeroreductase 1 HAMAP MF_00435
Alpha-keto-beta-hydroxylacil reductoisomerase 1 HAMAP MF_00435

Gene names

Name:	ilvC1 HAMAP MF_00435
Ordered Locus Names:	Noca_2140

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family. HAMAP MF_00435

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00435
Ligand	NADP HAMAP MF_00435
Molecular function	Isomerase EMBL ABL81649.1 Oxidoreductase HAMAP MF_00435 EMBL ABL81649.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	108	1	By similarity HAMAP MF_00435

Sequences

	Sequence		Length	Mass (Da)
	A1SIL4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 5412004692AB939E	FASTA	342	37,263
	10 20 30 40 50 60 MAEMFYDDDA DLSLIQGKNV AVIGYGSQGH AHALSLRDSG VDVRVGLQPG SKSRAKAEAE 70 80 90 100 110 120 GLRVVTPAEA AEEADVIVIL APDQHQRKLY AEEIEPALTP GDTLVFGHGF NIRFGYITPP 130 140 150 160 170 180 DGVDVFMVAP KGPGHLVRRE YVDGRGVPVL VAVEKDESGK AWNLALSYAK AIGGLRAGGI 190 200 210 220 230 240 RTTFTEETET DLFGEQAVLC GGASQLVMYG FEVLTEAGYQ PEVAYFECLH ELKLIVDLMY 250 260 270 280 290 300 EGGIAKQRWS CSDTAEFGDY LSGPRVITPD VKKNMEAVLA DIKNGSFAER FIGDMDNGSP 310 320 330 340 EFTEFRKKGE SHPIEQTGSE MRKLMSWVKS HDSDYTEGTA TR

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL81649.1.
RefSeq	YP_923336.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SIL4.
SMR	A1SIL4. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SIL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4599200.
GenomeReviews	Gene locus Noca_2140 in contig CP000509_GR.
KEGG	nca:Noca_2140.
PATRIC	22746321. VBINocSp122728_2404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HBG286525.
OMA	EYANGRG.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_2140-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1SIL4_NOCSJ

Accession

Primary (citable) accession number: A1SIL4

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)