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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Nocardioides sp. (strain BAA-499 / JS614)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei617 – 6171ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNSP196162:GH4V-1912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Noca_1884
OrganismiNocardioides sp. (strain BAA-499 / JS614)
Taxonomic identifieri196162 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPropionibacterialesNocardioidaceaeNocardioides
ProteomesiUP000000640 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 848848Leucine--tRNA ligasePRO_0000334783Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196162.Noca_1884.

Structurei

3D structure databases

ProteinModelPortaliA1SHV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionAdd
BLAST
Motifi614 – 6185"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1SHV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCPEQPHDTR AERDEMSEQT QQAAQPAETA AYDVRAIQDK WLPVWERLDP
60 70 80 90 100
FRADDSSPRE KKYALTMFPY PSGDLHMGHA EVTALHDVVA RYWWQRGYEV
110 120 130 140 150
LNPMGWDSFG LPAENAAIRN DEHPATYTYA NIETQYESFK RYAVSFDWSR
160 170 180 190 200
RLHTSDPEYY RWTQWLFLKF RERGLAYRKS SPVNWCPNDQ TVLANEQVVD
210 220 230 240 250
GRCERCGAEV TKRELTQWYF KITDYAQELL DRLDDLEPTW PARVVTAQRN
260 270 280 290 300
WIGRSEGAHV DFVVDGRDEP ITVYTTRPDT IFGTTFMVVA VDSPLAAELV
310 320 330 340 350
TDAQRPAFEA YREEIRKETE IERLSTDRPK TGVDLGVTAT NPVTGTQIPV
360 370 380 390 400
WATDYVLADY GTGAVMGVPG GDQRDWEFAT EMGLDIVRTT QTPEGFDGEA
410 420 430 440 450
YHGEGPAINS PAPGADAPLD INGLPVDEAK RATIEFLEQQ GTGAGAVNFR
460 470 480 490 500
LRDWLLSRQR YWGVPIPIIH CDACGEVAVP YDQLPLELPE LRGADLKPKG
510 520 530 540 550
VSPLAAAEEW VNVACPECGG AATRDSDTMD TFVDSSWYFL RYCSPDYTEG
560 570 580 590 600
PFDVEKAKAW MPADIYVGGV EHAVLHLLYA RFFTKVLRDM GMLEVDEPFA
610 620 630 640 650
AQLNQGIVIN QGKKMSKSLG NGVSLGDQLA EFGVDAVRVT LVFAGPPEDD
660 670 680 690 700
IDWADVSPAG ALRFLQRAWR LSGDVTSEAG TPAAGGDVAL RRVTARTVHD
710 720 730 740 750
AAELIESYRF NVMVARVMEL VNATRKAIDG APGPADPAVR EATETVAILL
760 770 780 790 800
SLVAPYVAEE MWERLGHEPT VARVGWPEVD PALLVEEQVT AVVQIQGKVR
810 820 830 840
ARLEVAPDIS EADLEQLAMA DPAVVRAIDG RPVRKVIVRA PKLVNVVV
Length:848
Mass (Da):94,337
Last modified:February 6, 2007 - v1
Checksum:i9FBB75346A8DB865
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL81394.1.
RefSeqiWP_011755341.1. NC_008699.1.
YP_923081.1. NC_008699.1.

Genome annotation databases

EnsemblBacteriaiABL81394; ABL81394; Noca_1884.
KEGGinca:Noca_1884.
PATRICi22745811. VBINocSp122728_2155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000509 Genomic DNA. Translation: ABL81394.1.
RefSeqiWP_011755341.1. NC_008699.1.
YP_923081.1. NC_008699.1.

3D structure databases

ProteinModelPortaliA1SHV9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196162.Noca_1884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL81394; ABL81394; Noca_1884.
KEGGinca:Noca_1884.
PATRICi22745811. VBINocSp122728_2155.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciNSP196162:GH4V-1912-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nocardioides sp. JS614."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F.
    , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BAA-499 / JS614.

Entry informationi

Entry nameiSYL_NOCSJ
AccessioniPrimary (citable) accession number: A1SHV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: June 24, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.