Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1SHV9 (SYL_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Noca_1884
OrganismNocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]
Taxonomic identifier196162 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides

Protein attributes

Sequence length848 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 848848Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334783

Regions

Motif69 – 7911"HIGH" region HAMAP-Rule MF_00049
Motif614 – 6185"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6171ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1SHV9 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 9FBB75346A8DB865

FASTA84894,337
        10         20         30         40         50         60 
MCPEQPHDTR AERDEMSEQT QQAAQPAETA AYDVRAIQDK WLPVWERLDP FRADDSSPRE 

        70         80         90        100        110        120 
KKYALTMFPY PSGDLHMGHA EVTALHDVVA RYWWQRGYEV LNPMGWDSFG LPAENAAIRN 

       130        140        150        160        170        180 
DEHPATYTYA NIETQYESFK RYAVSFDWSR RLHTSDPEYY RWTQWLFLKF RERGLAYRKS 

       190        200        210        220        230        240 
SPVNWCPNDQ TVLANEQVVD GRCERCGAEV TKRELTQWYF KITDYAQELL DRLDDLEPTW 

       250        260        270        280        290        300 
PARVVTAQRN WIGRSEGAHV DFVVDGRDEP ITVYTTRPDT IFGTTFMVVA VDSPLAAELV 

       310        320        330        340        350        360 
TDAQRPAFEA YREEIRKETE IERLSTDRPK TGVDLGVTAT NPVTGTQIPV WATDYVLADY 

       370        380        390        400        410        420 
GTGAVMGVPG GDQRDWEFAT EMGLDIVRTT QTPEGFDGEA YHGEGPAINS PAPGADAPLD 

       430        440        450        460        470        480 
INGLPVDEAK RATIEFLEQQ GTGAGAVNFR LRDWLLSRQR YWGVPIPIIH CDACGEVAVP 

       490        500        510        520        530        540 
YDQLPLELPE LRGADLKPKG VSPLAAAEEW VNVACPECGG AATRDSDTMD TFVDSSWYFL 

       550        560        570        580        590        600 
RYCSPDYTEG PFDVEKAKAW MPADIYVGGV EHAVLHLLYA RFFTKVLRDM GMLEVDEPFA 

       610        620        630        640        650        660 
AQLNQGIVIN QGKKMSKSLG NGVSLGDQLA EFGVDAVRVT LVFAGPPEDD IDWADVSPAG 

       670        680        690        700        710        720 
ALRFLQRAWR LSGDVTSEAG TPAAGGDVAL RRVTARTVHD AAELIESYRF NVMVARVMEL 

       730        740        750        760        770        780 
VNATRKAIDG APGPADPAVR EATETVAILL SLVAPYVAEE MWERLGHEPT VARVGWPEVD 

       790        800        810        820        830        840 
PALLVEEQVT AVVQIQGKVR ARLEVAPDIS EADLEQLAMA DPAVVRAIDG RPVRKVIVRA 


PKLVNVVV 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nocardioides sp. JS614."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BAA-499 / JS614.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000509 Genomic DNA. Translation: ABL81394.1.
RefSeqYP_923081.1. NC_008699.1.

3D structure databases

ProteinModelPortalA1SHV9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196162.Noca_1884.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL81394; ABL81394; Noca_1884.
GeneID4596385.
KEGGnca:Noca_1884.
PATRIC22745811. VBINocSp122728_2155.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycNSP196162:GH4V-1912-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NOCSJ
AccessionPrimary (citable) accession number: A1SHV9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries