Reviewed, UniProtKB/Swiss-Prot A1SG03 (T23O_NOCSJ)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Noca_1224

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Tryptophan 2,3-dioxygenase		PRO_0000360120
Regions
	Region	17 – 21	5	Substrate binding By similarity
	Region	44 – 48	5	Substrate binding By similarity
Sites
	Metal binding	225	1	Iron (heme axial ligand) By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	121	1	Heme By similarity
	Binding site	239	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SG03-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: BBDA339014A87854	FASTA	267	30,217
	10 20 30 40 50 60 MSDNFVSFGE QGAQLTYGSY LRLPQLLEAQ HLESDPPAHD ELLFITIHQV YELWFKQLLH 70 80 90 100 110 120 EVSAARDAML GGEAGGRLWW AQHLLTRVHV IERVLVQQID VLETMTPQEF LEFRQRLAPA 130 140 150 160 170 180 SGFQSVQFRE LEFLSGAKDP AYLERFRGIT PAEKARLDAR LSEPTLWDAF LAMLRSFGFA 190 200 210 220 230 240 ADSDAEVSAA LRTAAHDRTR YAVVWALSEG LLQHDELAAN WRARHVVMVE RMIGSKSGTG 250 260 GSSGSSYLRS RLPVQYYPLL WGLRSEL

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000509 Genomic DNA. Translation: ABL80738.1.
RefSeq	YP_922425.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1SG03.
Genome annotation databases
GeneID	4599273.
GenomeReviews	Gene locus Noca_1224 in contig CP000509_GR.
KEGG	nca:Noca_1224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QWSVLAT.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_NOCSJ

Accession

Primary (citable) accession number: A1SG03

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)