A1SF13 (KATG_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Noca_0875

Organism

Nocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]

Taxonomic identifier

196162 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Propionibacterineae › Nocardioidaceae › Nocardioides

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	Catalase-peroxidase HAMAP MF_01961		PRO_0000354852
Sites
	Active site	130	1	Proton acceptor By similarity
	Metal binding	292	1	Iron (heme axial ligand) By similarity
	Site	126	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	129 ↔ 251		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-277) By similarity
	Cross-link	251 ↔ 277		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-129) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1SF13 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6491CCF91EA4BCB3	FASTA	760	83,222
	10 20 30 40 50 60 MTDSQDNRTP ESPQGVDRKA EGGCPVLHDG VTAQGSESEN PAIDSPTPRT GGRPNSLKDW 70 80 90 100 110 120 WPNHLDLSVL HAHSSKSSPL DPGFRYSEEF EKLDIEALRR DIVEVLHTSQ DWWPADFGHY 130 140 150 160 170 180 GGLFVRMAWH AAGTYRIHDG RGGAGQGAQR FAPLNSWPDN ANLDKARRLL WPVKQKHGQK 190 200 210 220 230 240 ISWADLIVFA GNVALEDMGF TTFGFGFGRE DIWEPEEIYW GPEDTWLGDE RYSGDRELSN 250 260 270 280 290 300 PLGAVQMGLI YVNPEGPNGN PDPLASARDI RETFARMAMN DEETVALIAG GHTFGKTHGA 310 320 330 340 350 360 GDADLVGPEP EAAPLEAQGL GWHSSFGSGK GEDTITSGIE VTWTYHPTRW DNEFFHILYA 370 380 390 400 410 420 YEWELMKSAA GANQWRPKNG AGADMVPDAH DPSKRREPRM LTSDLALRFD PEYAKISSRF 430 440 450 460 470 480 KDHPEEFALA FAKAWYKLLH RDLGPIARYL GPLVGETQIW QDPVPAVDHE LVSDDDVAAL 490 500 510 520 530 540 KAKVLDSGLS VAELVSTAWA SAASFRSTDK RGGANGARIR LEPQRSWAVN QPEQLAGVLD 550 560 570 580 590 600 RLEGIQREFN EAGGAKISLA DLIVLAGSAA VEKAAKDGGV DVTVPFRAGR TDASQEQTDV 610 620 630 640 650 660 DSFAVLEPRA DGFRNYLLEN EKAQPEVLLV ERAYLLGLTA PEMTVLVGGL RALGNNVGGS 670 680 690 700 710 720 GHGVLTDRPG VLTNDFFANL LAPGAQWKAS ESEANVYEIR DLASGELRWT ATAVDLIFGS 730 740 750 760 NSQLRSLAEV YASADAREKF VRDFVAAWVK VMDADRFDLA

References

[1]

"Complete sequence of chromosome 1 of Nocardioides sp. JS614." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAA-499 / JS614.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000509 Genomic DNA. Translation: ABL80398.1.
RefSeq	YP_922085.1. NC_008699.1.
3D structure databases
ProteinModelPortal	A1SF13.
SMR	A1SF13. Positions 56-760.
ModBase	Search...
Protein-protein interaction databases
STRING	A1SF13.
Protein family/group databases
PeroxiBase	2691. NspCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4599882.
GenomeReviews	Gene locus Noca_0875 in contig CP000509_GR.
KEGG	nca:Noca_0875.
PATRIC	22743745. VBINocSp122728_1132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	A1SF13.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	NSP35761:NOCA_0875-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_NOCSJ

Accession

Primary (citable) accession number: A1SF13

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program