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A1SE40 (NUON_NOCSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit N

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit N
NDH-1 subunit N
Gene names
Name:nuoN
Ordered Locus Names:Noca_0533
OrganismNocardioides sp. (strain BAA-499 / JS614) [Complete proteome] [HAMAP]
Taxonomic identifier196162 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaeNocardioidaceaeNocardioides

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_00445

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_00445

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00445.

Sequence similarities

Belongs to the complex I subunit 2 family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533NADH-quinone oxidoreductase subunit N HAMAP-Rule MF_00445
PRO_0000391191

Regions

Transmembrane13 – 3321Helical; Potential
Transmembrane40 – 6021Helical; Potential
Transmembrane87 – 10721Helical; Potential
Transmembrane141 – 16121Helical; Potential
Transmembrane164 – 18421Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane243 – 26321Helical; Potential
Transmembrane275 – 29521Helical; Potential
Transmembrane310 – 33021Helical; Potential
Transmembrane337 – 35721Helical; Potential
Transmembrane373 – 39321Helical; Potential
Transmembrane417 – 43721Helical; Potential
Transmembrane451 – 47121Helical; Potential
Transmembrane502 – 52221Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
A1SE40 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 68DF5F7C1A0048E7

FASTA53356,131
        10         20         30         40         50         60 
MDFIKPTIEY GDVWPLLVVF GVAAVGVLVE GFVPRAQRYL VQAALAIAGV VVALVGTILV 

        70         80         90        100        110        120 
ARDLDVLGDG AARGAIDVEG TIAVDGPALF IWGMLLVFAL GGALLFAERR LEGGVSAFAG 

       130        140        150        160        170        180 
QAAALPGTEA ERQASTRGLE HTEVYPLMMF ALGGMMLFAA ANDLLTLFVA LEVLSLPLYL 

       190        200        210        220        230        240 
LSGLARRRRL LSQEAALKYF MLGAFSSGFF LYGAALVYGF SGSMGFAEIN EAVRDDVGNQ 

       250        260        270        280        290        300 
TLLLIGIGML SVGLLFKVGA VPFHSWTPDV YQGAPTAVTA FMAAGTKIAA FGAMLRLFYV 

       310        320        330        340        350        360 
AFGSDRWSWQ PMLWIIAILT MLVGALIAIV QTDMKRMLAY SSVAHTGFLL TGVLGVQQAS 

       370        380        390        400        410        420 
ELADGEVTSL QAVLFYLVTY GFAVVGAFAV VTLVRDAGGE AGQFVRWRGI GRRSPLVAGV 

       430        440        450        460        470        480 
FAFFLLSMAG IPLTAGFVGK WAVFTVALAA GAWPVVIAAV LCSIIAVFFY VRVILLMFFE 

       490        500        510        520        530 
DDDVSAQGEV ASVTKPSVLT SATIFVGVAA TLVLGVVPGP VLDLAANAGQ FVR 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nocardioides sp. JS614."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Thompson L.S., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Mattes T., Gossett J., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BAA-499 / JS614.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000509 Genomic DNA. Translation: ABL80075.1.
RefSeqYP_921762.1. NC_008699.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196162.Noca_0533.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL80075; ABL80075; Noca_0533.
GeneID4596200.
KEGGnca:Noca_0533.
PATRIC22743083. VBINocSp122728_0807.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1007.
HOGENOMHOG000100794.
KOK00343.
OMASIVQLAM.
OrthoDBEOG64JFNZ.

Enzyme and pathway databases

BioCycNSP196162:GH4V-544-MONOMER.

Family and domain databases

HAMAPMF_00445. NDH1_NuoN_1.
InterProIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01770. NDH_I_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNUON_NOCSJ
AccessionPrimary (citable) accession number: A1SE40
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families